Register Janna K, Fales Andrew M, Wang Hsin-Neng, Norton Stephen J, Cho Eugenia H, Boico Alina, Pradhan Sulolit, Kim Jason, Schroeder Thies, Wisniewski Natalie A, Klitzman Bruce, Vo-Dinh Tuan
Fitzpatrick Institute for Photonics, Departments of Biomedical Engineering and Chemistry, Duke University, 136 Hudson Hall, Box 90281, Durham, NC, 27708, USA.
Medical Center, Duke University, 201 Trent Dr, Durham, NC, 27710, USA.
Anal Bioanal Chem. 2015 Nov;407(27):8215-24. doi: 10.1007/s00216-015-8939-0. Epub 2015 Sep 4.
Surface-enhanced Raman scattering (SERS)-active plasmonic nanomaterials have become a promising agent for molecular imaging and multiplex detection. Among the wide variety of plasmonics-active nanoparticles, gold nanostars offer unique plasmon properties that efficiently induce strong SERS signals. Furthermore, nanostars, with their small core size and multiple long thin branches, exhibit high absorption cross sections that are tunable in the near-infrared region of the tissue optical window, rendering them efficient for in vivo spectroscopic detection. This study investigated the use of SERS-encoded gold nanostars for in vivo detection. Ex vivo measurements were performed using human skin grafts to investigate the detection of SERS-encoded nanostars through tissue. We also integrated gold nanostars into a biocompatible scaffold to aid in performing in vivo spectroscopic analyses. In this study, for the first time, we demonstrate in vivo SERS detection of gold nanostars using small animal (rat) as well as large animal (pig) models. The results of this study establish the usefulness and potential of SERS-encoded gold nanostars for future use in long-term in vivo analyte sensing.
表面增强拉曼散射（SERS）活性等离子体纳米材料已成为分子成像和多重检测的一种有前景的试剂。在各种各样的等离子体活性纳米颗粒中，金纳米星具有独特的等离子体特性，能够有效地诱导出强烈的SERS信号。此外，纳米星具有小的核心尺寸和多个细长的分支，表现出高吸收截面，且在组织光学窗口的近红外区域是可调谐的，这使得它们在体内光谱检测中效率很高。本研究调查了SERS编码的金纳米星在体内检测中的应用。使用人类皮肤移植进行了体外测量，以研究通过组织对SERS编码的纳米星的检测。我们还将金纳米星整合到生物相容性支架中，以辅助进行体内光谱分析。在本研究中，我们首次使用小动物（大鼠）和大动物（猪）模型证明了金纳米星的体内SERS检测。本研究结果确立了SERS编码的金纳米星在未来长期体内分析物传感中的实用性和潜力。
