将感染样本中的病毒信号直接应用于硝酸纤维素膜进行原位扩增。
In situ amplification of viral signal in infected samples applied directly to nitrocellulose membranes.
作者信息
De Stasio P R, Etchberger K J, Paeratakul U, Taylor M W
机构信息
Department of Biology, Indiana University, Bloomington 47405.
出版信息
Biotechniques. 1989 May;7(5):442-7.
PMID:2633792
Abstract
A method for the amplification of viral signal present in infected samples is described. Viral suspensions are spotted on nitrocellulose paper, and immediately afterwards an adequate amount of a permissive cell line is added. The nitrocellulose filter is then incubated overnight, fixed and hybridized to labeled viral probes. The technique is extremely fast, reproducible and inexpensive, and may be readily applicable to the clinical diagnosis of many viral diseases.
摘要
本文描述了一种用于扩增感染样本中病毒信号的方法。将病毒悬液点样于硝酸纤维素纸上，随后立即加入适量的易感细胞系。然后将硝酸纤维素滤膜孵育过夜，固定并与标记的病毒探针杂交。该技术极其快速、可重复且成本低廉，并且可轻易应用于多种病毒性疾病的临床诊断。
Zotero 插件