NutriFOODchem Unit, Department of Food Safety and Food Quality (Partner in Food2Know), Faculty of Bioscience Engineering, Ghent University, Belgium; Laboratory of Food Analysis, Department of Bioanalysis (Partner in Food2Know), Faculty of Pharmaceutical Sciences, Ghent University, Belgium.
Laboratory of Food Microbiology and Food Preservation, Department of Food Safety and Food Quality (Partner in Food2Know), Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000, Ghent, Belgium.
Food Microbiol. 2015 Dec;52:185-96. doi: 10.1016/j.fm.2015.07.016. Epub 2015 Jul 26.
A liquid chromatography triple quadrupole tandem mass spectrometry method was developed and validated to determine mycotoxins, produced by fungal isolates grown on malt extract agar (MEA). All twenty metabolites produced by different fungal species were extracted using acetonitrile/1% formic acid. The developed method was applied to assess the toxigenic potentiality of Aspergillus flavus (n = 11) and Aspergillus parasiticus (n = 6) strains isolated from black peppers (Piper nigrum L.) following their growth at 22, 30 and 37 °C. Highest mean radial colony growth rates were observed at 30 °C for A. flavus (5.21 ± 0.68 mm/day) and A. parasiticus (4.97 ± 0.33 mm/day). All of the A. flavus isolates produced aflatoxin B1 and O-methyl sterigmatocystin (OMST) while 91% produced aflatoxin B2 (AFB2) and 82% of them produced sterigmatocystin (STERIG) at 30 °C. Except one, all the A. parasiticus isolates produced all the four aflatoxins, STERIG and OMST at 30 °C. Remarkably high AFB1 was produced by some A. flavus isolates at 22 °C (max 16-40 mg/kg). Production of mycotoxins followed a different trend than that of growth rate of both species. Notable correlations were found between different secondary metabolites of both species; R(2) 0.87 between AFB1 and AFB2 production. Occurrence of OMST could be used as a predictor for AFB1 production.
建立并验证了一种基于液相色谱三重四级杆串联质谱法的方法，用于检测在麦芽提取物琼脂（MEA）上生长的真菌分离物产生的霉菌毒素。使用乙腈/1%甲酸提取不同真菌物种产生的全部二十种代谢产物。该方法用于评估从黑胡椒（Piper nigrum L.）中分离出的黄曲霉（n=11）和寄生曲霉（n=6）菌株的产毒潜力，这些菌株在 22、30 和 37°C 下生长。黄曲霉（A. flavus）（5.21±0.68mm/天）和寄生曲霉（A. parasiticus）（4.97±0.33mm/天）在 30°C 时的平均径向菌落生长速率最高。所有黄曲霉分离株均产生黄曲霉 B1 和 O-甲基杂色曲菌素（OMST），而 91%的分离株在 30°C 时产生黄曲霉 B2（AFB2），82%的分离株产生杂色曲菌素（STERIG）。除一个外，所有寄生曲霉分离株均在 30°C 时产生四种黄曲霉毒素、STERIG 和 OMST。一些黄曲霉分离株在 22°C 时产生了非常高的 AFB1（最高达 16-40mg/kg）。两种真菌的产毒情况与生长速率不同。两种真菌的不同次级代谢产物之间存在显著相关性；AFB1 和 AFB2 产量之间的 R2 为 0.87。OMST 的存在可用作 AFB1 产量的预测因子。
