Gonçalves Tatiana Siqueira, Menezes Luciane Macedo de, Trindade Cristiano, Thomas Philip, Fenechc Michael, Henriques João Antonio Pêgas
Department of Orthodontics, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Programa de Pós Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
Mutat Res Genet Toxicol Environ Mutagen. 2015 Sep;791:25-9. doi: 10.1016/j.mrgentox.2015.07.007. Epub 2015 Jul 23.
Auxiliary appliances consisting of silver-soldered joints may be present in the patient's oral cavity for a long time. The aim of this study was to investigate, in vivo, the potential genotoxic effects of Hyrax-type maxillary expanders containing silver-soldered joints on the cells of the buccal mucosa. Buccal cells were collected from 20 patients and processed to perform the buccal comet assay (BCA) and the buccal micronucleus cytome (BMCyt) assay, to investigate DNA and chromosomal damage, respectively. For the BCA, patients were evaluated before and 14 days after the appliances were installed. For the BMCyt assay, the patients were evaluated longitudinally; before the insertion of the appliance and after one, six, and twelve months. The BCA showed significant increases in damage frequency and damage index in exfoliated buccal cells, following insertion of the appliance. For the BMCyt assay, the endpoints related to cell proliferation, cell death, and chromosomal damage (micronuclei and nuclear buds) resulted in no significant differences over the 12-month study period. In conclusion, the use of orthodontic appliances containing silver-soldered joints can significantly increase DNA damage as measured by the BCA.
患者口腔内可能长期存在由银焊连接组成的辅助器具。本研究的目的是在体内研究含银焊连接的Hyrax型上颌扩弓器对颊黏膜细胞的潜在遗传毒性作用。从20名患者收集颊细胞，并进行处理以分别进行颊部彗星试验（BCA）和颊部微核细胞试验（BMCyt），以研究DNA和染色体损伤。对于BCA，在装置安装前和安装后14天对患者进行评估。对于BMCyt试验，对患者进行纵向评估；在装置插入前以及插入后1个月、6个月和12个月进行评估。BCA显示，装置插入后，脱落颊细胞的损伤频率和损伤指数显著增加。对于BMCyt试验，在12个月的研究期内，与细胞增殖、细胞死亡和染色体损伤（微核和核芽）相关的终点未发现显著差异。总之，使用含银焊连接的正畸矫治器可显著增加BCA检测到的DNA损伤。
