Singh Neetu, Chaudhary Anshu, Singh Hridaya Shanker
Molecular Taxonomy Laboratory, Department of Zoology, University Road, Chaudhary Charan Singh University, Meerut (UP), 250004, India.
Bioinformation. 2015 Jul 31;11(7):343-7. doi: 10.6026/97320630011343. eCollection 2015.
In this study, we sequenced mt Cox 1 gene sequences of five nematode spp. that were infective to arthropod, Gryllotalpa africana. The nematode belongs to Thelastomatoidea, a group of pinworms that parasitizes only invertebrates. Currently, in India spp. of this group are distinguished mainly on the basis of morphological characters that present possible confusions. Therefore, we identified the species through morphological and genetic analysis. We selected mt Cox 1 gene region to show their phylogenetic position with closely related spp. and confirmed their molecular validation. The present findings are important to confirm the phylogenetic position and relationship among five nematode spp. and avoid misidentification regarding their validation, as it is more necessary in that case when many species harbours the same host.
在本研究中，我们对五种可感染节肢动物非洲蝼蛄的线虫物种的线粒体细胞色素c氧化酶亚基1（mt Cox 1）基因序列进行了测序。这些线虫属于后圆线虫总科，是一类仅寄生于无脊椎动物的蛲虫。目前，在印度，该类群的物种主要基于形态特征进行区分，这可能会导致混淆。因此，我们通过形态学和遗传学分析来鉴定这些物种。我们选择mt Cox 1基因区域来显示它们与近缘物种的系统发育位置，并确认它们的分子验证。目前的研究结果对于确认这五种线虫物种的系统发育位置和关系以及避免在其验证方面的错误鉴定非常重要，因为在许多物种共享同一宿主的情况下，这一点更为必要。
