Kollars Nicole M, Krueger-Hadfield Stacy A, Byers James E, Greig Thomas W, Strand Allan E, Weinberger Florian, Sotka Erik E
Grice Marine Laboratory and the Department of Biology, College of Charleston, Charleston, SC, USA.
Current affiliation: Center for Population Biology, University of California, Davis, CA, USA.
PeerJ. 2015 Aug 11;3:e1159. doi: 10.7717/peerj.1159. eCollection 2015.
Microsatellite loci are popular molecular markers due to their resolution in distinguishing individual genotypes. However, they have rarely been used to explore the population dynamics in species with biphasic life cycles in which both haploid and diploid stages develop into independent, functional organisms. We developed microsatellite loci for the haploid-diploid red seaweed Gracilaria vermiculophylla, a widespread non-native species in coastal estuaries of the Northern hemisphere. Forty-two loci were screened for amplification and polymorphism. Nine of these loci were polymorphic across four populations of the extant range with two to eleven alleles observed. Mean observed and expected heterozygosities ranged from 0.265 to 0.527 and 0.317 to 0.387, respectively. Overall, these markers will aid in the study of the invasive history of this seaweed and further studies on the population dynamics of this important haploid-diploid primary producer.
微卫星位点因其在区分个体基因型方面的分辨率而成为常用的分子标记。然而，它们很少被用于探索具有双相生命周期的物种的种群动态，在这种生命周期中，单倍体和二倍体阶段都会发育成独立的、有功能的生物体。我们为北半球沿海河口广泛分布的非本地物种——单倍体-二倍体红藻细基江蓠（Gracilaria vermiculophylla）开发了微卫星位点。筛选了42个位点用于扩增和多态性分析。其中9个位点在现存分布范围的四个种群中具有多态性，观察到2至11个等位基因。观察到的平均杂合度和期望杂合度分别在0.265至0.527和0.317至0.387之间。总体而言，这些标记将有助于研究这种海藻的入侵历史，并进一步研究这种重要的单倍体-二倍体初级生产者的种群动态。
