Biosens Bioelectron. 2016 Jan 15;75:365-74. doi: 10.1016/j.bios.2015.08.058.
We have developed a lab-on-a-chip (LOC) platform for electrochemical detection and degradation of the pesticide atrazine (Atz). It is based on boron-doped diamond (BDD) electrodes and a competitive magneto-enzyme immunoassay (EIA) that enables high sensitivity. To detect the enzymatic reaction, we employed a BDD electrode modified with platinum nanoparticles (PtNPs), as a highly conductive catalytic transducer. Chronoamperometry revealed a limit of detection (LOD) of 3.5 pM for atrazine, which, to the best of our knowledge, is one of the lowest value published to date. Finally, we degraded Atz in the same platform, using a bare BDD electrode that features remarkable corrosion stability, a wide potential window, and much higher O2 overvoltage as compared to conventional electrodes. These characteristics enable the electrode to produce a greater amount of HO• on the anode surface than do conventional electrodes and consequently, to destroy the pollutant more rapidly. Our new LOC platform might prove interesting as a smart system for detection and remediation of diverse pesticides and other contaminants.
我们开发了一种用于电化学生物传感器检测和降解农药莠去津的片上实验室（LOC）平台。该平台基于掺硼金刚石（BDD）电极和竞争型磁酶免疫分析（EIA），具有高灵敏度。为了检测酶反应，我们采用了铂纳米粒子（PtNPs）修饰的 BDD 电极，作为一种高导电性的催化换能器。计时安培法显示莠去津的检测限（LOD）为 3.5 pM，据我们所知，这是迄今为止报道的最低值之一。最后，我们在相同的平台上使用裸 BDD 电极降解莠去津，该电极具有出色的耐腐蚀性、宽电位窗口以及比传统电极更高的 O2 过电压。这些特性使得电极在阳极表面产生更多的 HO•，从而更快速地破坏污染物。我们的新 LOC 平台可能会成为一种用于检测和修复各种农药和其他污染物的智能系统。
