Pino Flavio, Ivandini Tribidasari A, Nakata Kazuya, Fujishima Akira, Merkoçi Arben, Einaga Yasuaki
Nanobioelectronics & Biosensors Group, ICN2 - Institut Català de Nanociencia i Nanotecnologia (CERCA-CSIC-UAB).
Anal Sci. 2015;31(10):1061-8. doi: 10.2116/analsci.31.1061.
A simple and reliable enzymatic system for organophosporus pesticide detection was successfully developed, by exploiting the synergy between the magnetic beads collection capacity and the outstanding electrochemistry property of boron-doped diamond electrodes. The determination of an organophosphate pesticide, chlorpyrifos (CPF), was performed based on the inhibition system of the enzyme acetylcholinesterase bonded to magnetic beads through a biotin-streptavidin complex system. A better sensitivity was found for a system with magnetic beads in the concentration range of 10(-9) to 10(-5) M. The estimated limits of detection based on IC10 (10% acetylcholinesterase (AChE) inhibition) have been detected and optimized to be 5.7 × 10(-10) M CPF. Spiked samples of water of Yokohama (Japan) have been measured to validate the efficiency of the enzymatic system. The results suggested that the use of magnetic beads to immobilize biomolecules or biosensing agents is suitable to maintain the superiority of BDD electrodes.
通过利用磁珠的捕获能力与硼掺杂金刚石电极出色的电化学性能之间的协同作用，成功开发了一种用于有机磷农药检测的简单可靠的酶促系统。基于通过生物素-链霉亲和素复合系统与磁珠结合的乙酰胆碱酯酶的抑制系统，对有机磷农药毒死蜱（CPF）进行了测定。对于磁珠浓度在10（-9）至10（-5）M范围内的系统，发现了更好的灵敏度。基于IC10（10%乙酰胆碱酯酶（AChE）抑制）检测并优化后的估计检测限为5.7×10（-10）M CPF。对日本横滨的加标水样进行了测量，以验证该酶促系统的效率。结果表明，使用磁珠固定生物分子或生物传感剂适合保持BDD电极的优势。
