Werner Guido, Fleige Carola, Neumann Bernd, Bender Jennifer K, Layer Franziska, Klare Ingo
Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany; Institute for Microbiology, Ernst-Moritz-Arndt-University, Greifswald, Germany.
J Microbiol Methods. 2015 Nov;118:81-4. doi: 10.1016/j.mimet.2015.08.019. Epub 2015 Sep 1.
We evaluated and critically assessed the performance and discriminatory power of a rep-PCR based commercial test DiversiLab® Enterococcus kit (bioMerieux) for typing a set of 65 representative isolates of Enterococcus faecium/VRE and compared it to state-of-the-art typing techniques such as PFGE and MLST.
我们评估并严格评估了基于rep-PCR的商业检测试剂盒DiversiLab®肠球菌试剂盒(生物梅里埃公司)对一组65株粪肠球菌/耐万古霉素肠球菌代表性分离株进行分型的性能和鉴别能力,并将其与诸如脉冲场凝胶电泳(PFGE)和多位点序列分型(MLST)等先进分型技术进行了比较。