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韩国加工猪肉制品中[具体微生物名称未给出]的发生情况、抗菌药物耐药性及分子多样性

Occurrence, Antimicrobial Resistance and Molecular Diversity of in Processed Pork Meat Products in Korea.

作者信息

Kim Hyun Jung, Koo Minseon

机构信息

Research Group of Consumer Safety, Korea Food Research Institute, Wanju, Jeollabuk-do 55365, Korea.

Department of Food Biotechnology, University of Science and Technology, Daejeon 34113, Korea.

出版信息

Foods. 2020 Sep 12;9(9):1283. doi: 10.3390/foods9091283.

Abstract

Because is an important nosocomial pathogen and sentinel organism for tracking antimicrobial resistance, information on the contamination and antimicrobial resistance patterns of in food are essential to public health and food safety. We analyzed the occurrence of in retail pork meat products ( = 124), and antimicrobial resistance of 30 isolates were examined against 14 antimicrobials using the broth dilution test and disc diffusion test. Rep-PCR-based molecular diversity was also analyzed using Deviersilab. The highest contamination of enterococci was observed for minced pork meat but most of the was isolated from meatball-type frozen pork meat products (FP). Incidences of antimicrobial-resistant against erythromycin, clindamycin and nitrofurantoin were 80%, 50% and 20%, respectively. No vancomycin-resistant enterococci were analyzed. Rep-PCR showed distinctive clusters with a similarity ≥ 98%, consisting of 18 isolates from FP manufactured in seven companies. The analyzed data on the contamination and antimicrobial resistance patterns combined with molecular typing can be useful to derive risk management of antimicrobial-resistant enterococci in food.

摘要

由于[某种细菌名称]是一种重要的医院病原体和追踪抗菌药物耐药性的指示生物,因此关于其在食品中的污染情况和抗菌药物耐药模式的信息对于公共卫生和食品安全至关重要。我们分析了零售猪肉制品(n = 124)中[某种细菌名称]的存在情况,并使用肉汤稀释试验和纸片扩散试验检测了30株[某种细菌名称]分离株对14种抗菌药物的耐药性。还使用Deviersilab分析了基于重复聚合酶链反应(Rep-PCR)的分子多样性。碎猪肉中观察到肠球菌的污染率最高,但大多数[某种细菌名称]分离自肉丸型冷冻猪肉制品(FP)。对红霉素、克林霉素和呋喃妥因耐药的[某种细菌名称]的发生率分别为80%、50%和20%。未分析万古霉素耐药肠球菌。Rep-PCR显示出相似性≥98%的独特聚类,由来自7家公司生产的FP中的18株[某种细菌名称]分离株组成。结合分子分型对污染情况和抗菌药物耐药模式进行的分析数据,有助于制定食品中抗菌药物耐药肠球菌的风险管理措施。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae8/7555021/4b8a9791434d/foods-09-01283-g001.jpg

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