Caglar Aytac Pinar, Kilicdag Esra Bulgan, Haydardedeoglu Bulent, Simsek Erhan, Cok Tayfun, Parlakgumus Huriye Ayse
Division of Reproductive Endocrinology and IVF Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Baskent University, Adana, Turkey.
Division of Reproductive Endocrinology and IVF Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Baskent University, Adana, Turkey.
Fertil Steril. 2015 Nov;104(5):1168-74. doi: 10.1016/j.fertnstert.2015.07.1163. Epub 2015 Sep 3.
To determine whether calcium ionophore solution can improve the fertilization rate in patients with diminished ovarian reserve whose partners have normal sperm parameters.
Between January 2014 and August 2014, patients with diminished ovarian reserve were randomized to make artificial oocyte activation with calcium ionophore solution.
University hospital.
PATIENT(S): A total of 296 patients who had diminished ovarian reserve and partners with normal sperm parameters were included in the study.
INTERVENTION(S): Metaphase 2 oocytes were treated with calcium ionophore solution (GM508 Cult-Active) for 15 minutes just after intracytoplasmic sperm injection.
MAIN OUTCOME MEASURE(S): Fertilization rate, implantation rate, clinical pregnancy rate, ongoing pregnancy rate.
RESULT(S): Fertilization, implantation, pregnancy, and ongoing pregnancy rates for the calcium ionophore and control groups were 60.7% and 55.4%, 12.8% and 10.7%, 21% and 12.8%, and 10.9% and 6.1%, respectively.
CONCLUSION(S): This is the first prospective, randomized, controlled study to analyze the effect of calcium ionophore solution on fertilization rate in patients with diminished ovarian reserve. We did not observe any differences in fertilization, clinical pregnancy, or ongoing pregnancy rates between the groups. We propose that fertilization ratios could not be increased by artificial oocyte activation via application of calcium ionophore solution in patients with diminished ovarian reserve.
NCT02045914.
确定钙离子载体溶液能否提高卵巢储备功能减退且其配偶精子参数正常患者的受精率。
2014年1月至2014年8月期间，将卵巢储备功能减退的患者随机分组，采用钙离子载体溶液进行人工卵母细胞激活。
大学医院。
共有296例卵巢储备功能减退且配偶精子参数正常的患者纳入本研究。
卵母细胞胞浆内单精子注射后，用钙离子载体溶液（GM508 Cult-Active）对处于减数分裂中期Ⅱ的卵母细胞处理15分钟。
受精率、着床率、临床妊娠率、持续妊娠率。
钙离子载体组和对照组的受精率、着床率、妊娠率和持续妊娠率分别为60.7%和55.4%、12.8%和10.7%、21%和12.8%、10.9%和6.1%。
这是第一项前瞻性、随机、对照研究，旨在分析钙离子载体溶液对卵巢储备功能减退患者受精率的影响。我们未观察到两组在受精率、临床妊娠率或持续妊娠率方面存在任何差异。我们认为，对于卵巢储备功能减退的患者，通过应用钙离子载体溶液进行人工卵母细胞激活并不能提高受精率。
NCT02045914。
