通过反相色谱法分离DNA序列中的四链体多态性
Separation of Quadruplex Polymorphism in DNA Sequences by Reversed-Phase Chromatography.
作者信息
Miller M Clarke, Ohrenberg Carl J, Kuttan Ashani, Trent John O
机构信息
Department of Chemistry and Biochemistry, University of North Georgia, Oakwood, Georgia.
James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky.
出版信息
Curr Protoc Nucleic Acid Chem. 2015 Jun 1;61:17.7.1-17.7.18. doi: 10.1002/0471142700.nc1707s61.
This unit describes a method for the separation of a mixture of quadruplex conformations formed from the same parent sequence via reversed-phase chromatography (RPC). Polymorphism is inherent to quadruplex formation and even relatively simple quadruplex-forming sequences can fold into a cornucopia of possible conformations and topologies. Isolation of a specific conformation for study can be problematic. This is especially true for conformations of the human telomere sequence d(GGG(TTAGGG)3). High performance liquid chromatography (HPLC), especially reversed-phase chromatography, has been a mainstay of nucleic acid research and purification for many decades. We have successfully applied this method to the problem of separating individual quadruplex species in the ensemble from the same parent sequence.
本单元描述了一种通过反相色谱法（RPC）分离由相同母序列形成的四链体构象混合物的方法。多态性是四链体形成所固有的，即使是相对简单的四链体形成序列也可以折叠成大量可能的构象和拓扑结构。分离特定构象用于研究可能会有问题。对于人类端粒序列d(GGG(TTAGGG)3)的构象来说尤其如此。几十年来，高效液相色谱法（HPLC），尤其是反相色谱法，一直是核酸研究和纯化的主要手段。我们已成功将此方法应用于从相同母序列的集合中分离单个四链体物种的问题。
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