从圆二色性光谱获得的 G-四链体二级结构。
G-Quadruplex Secondary Structure Obtained from Circular Dichroism Spectroscopy.
机构信息
James Graham Brown Cancer Center, University of Louisville, 505 S. Hancock St., Louisville, KY, 40202, USA.
出版信息
Angew Chem Int Ed Engl. 2018 Jun 11;57(24):7171-7175. doi: 10.1002/anie.201709184. Epub 2018 Apr 25.
Abstract
A curated library of circular dichroism spectra of 23 G-quadruplexes of known structure was built and analyzed. The goal of this study was to use this reference library to develop an algorithm to derive quantitative estimates of the secondary structure content of quadruplexes from their experimental CD spectra. Principal component analysis and singular value decomposition were used to characterize the reference spectral library. CD spectra were successfully fit to obtain estimates of the amounts of base steps in anti-anti, syn-anti or anti-syn conformations, in diagonal or lateral loops, or in other conformations. The results show that CD spectra of nucleic acids can be analyzed to obtain quantitative structural information about secondary structure content in an analogous way to methods used to analyze protein CD spectra.
摘要
建立并分析了一个经过精心挑选的包含 23 个已知结构的 G-四链体的圆二色性光谱库。本研究的目的是利用这个参考文库开发一种算法，从 G-四链体的实验 CD 光谱中得出其二级结构含量的定量估计。主成分分析和奇异值分解用于描述参考光谱库。成功地对 CD 光谱进行拟合，以获得反式反式、顺式反式或反式顺式构象、对角或侧向环或其他构象中碱基步的量的估计。结果表明，可以对 CD 光谱进行分析，以类似于分析蛋白质 CD 光谱的方法，获得有关二级结构含量的定量结构信息。
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