季铵化壳聚糖和大豆分离蛋白自组装纳米纤维垫的抗菌应用。
Antimicrobial application of nanofibrous mats self-assembled with quaternized chitosan and soy protein isolate.
机构信息
Department of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China; College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
出版信息
Carbohydr Polym. 2015 Nov 20;133:229-35. doi: 10.1016/j.carbpol.2015.07.019. Epub 2015 Jul 13.
Positively charged N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTCC) and negatively charged soy protein isolate (SPI) were alternately assembled on cellulose acetate (CA) electrospun nanofibrous mats via electrostatic layer-by-layer self-assembly technique. CA nanofibrous mats coated with bilayers of HTCC and SPI possessed more orderly-arranged structure than uncoated CA mats according to the observation from scanning electron microscopy images. The average diameter of the nanofibers was enlarged by the increase of the bilayer number. X-ray photoelectron spectroscopy indicated that HTCC and SPI were coated on the surface of the CA mats successfully. The average diameters of inhibition zones of (HTCC/SPI)10.5-films-coated nanofibrous mats against Escherichia coli and Staphylococcus aureus were 9.6mm and 11.53mm, respectively, which demonstrated the highest antimicrobial activity among samples in presented study.
正电荷 N-[(2-羟基-3-三甲基铵)丙基]壳聚糖氯化物（HTCC）和带负电荷的大豆分离蛋白（SPI）通过静电层层自组装技术在醋酸纤维素（CA）电纺纳米纤维垫上交替组装。扫描电子显微镜图像观察表明，与未涂层 CA 垫相比，涂有 HTCC 和 SPI 双层的 CA 纳米纤维垫具有更有序排列的结构。随着双层数量的增加，纳米纤维的平均直径增大。X 射线光电子能谱表明 HTCC 和 SPI 成功地涂覆在 CA 垫的表面。（HTCC/SPI）10.5 薄膜涂层纳米纤维垫对大肠杆菌和金黄色葡萄球菌的抑菌圈平均直径分别为 9.6mm 和 11.53mm，在本研究中表现出最高的抗菌活性。
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