Chen T L, Mallory J B, Chang S L
California Biotechnology Inc., Mountain View, California 94043.
Growth Factors. 1989;1(4):335-45. doi: 10.3109/08977198909000257.
The present study examines how the mitogenic and differentiation functions of transforming growth factor-beta (TGF-beta) are modulated by basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) in primary cultures of rat osteoblast-like (ROB) cells. TGF-beta, bFGF, and EGF individually stimulated [3H]thymidine incorporation and cell proliferation in a dose range of 0.01-10 ng/ml. When studied in combination, high doses of bFGF and EGF were additive to low doses of TGF-beta. The additive effects of bFGF and EGF on mitogenesis diminished with increasing doses of TGF-beta. These three factors also decreased alkaline phosphatase activity individually within the same dose range. When cells were treated with the combined factors, only high doses of bFGF and EGF were additive to the TGF-beta inhibition. We were unable to detect any change in collagen synthesis with each individual factor or in combined treatments. In addition, TGF-beta or bFGF alone or in combination did not affect fibronectin synthesis. Our studies showed that the biological functions of TGF-beta can be modulated by bFGF and EGF in ROB cells. The pattern of modulation is varied depending on the specific function examined.
本研究探讨了在大鼠成骨样（ROB）细胞原代培养中，碱性成纤维细胞生长因子（bFGF）和表皮生长因子（EGF）如何调节转化生长因子-β（TGF-β）的促有丝分裂和分化功能。TGF-β、bFGF和EGF在0.01-10 ng/ml的剂量范围内均可单独刺激[3H]胸腺嘧啶核苷掺入和细胞增殖。联合研究时，高剂量的bFGF和EGF与低剂量的TGF-β具有相加作用。bFGF和EGF对有丝分裂的相加作用随TGF-β剂量增加而减弱。这三种因子在相同剂量范围内也均可单独降低碱性磷酸酶活性。当细胞用联合因子处理时，只有高剂量的bFGF和EGF与TGF-β的抑制作用具有相加性。我们未检测到单独使用每种因子或联合处理时胶原蛋白合成有任何变化。此外，单独或联合使用TGF-β或bFGF均不影响纤连蛋白合成。我们的研究表明，TGF-β的生物学功能可在ROB细胞中被bFGF和EGF调节。调节模式因所检测的特定功能而异。
