Ohji M, SundarRaj N, Thoft R A
Department of Ophthalmology, University of Pittsburgh, PA.
Curr Eye Res. 1993 Aug;12(8):703-9. doi: 10.3109/02713689308995765.
The effects of transforming growth factor-beta (TGF beta) and epidermal growth factor (EGF) on the synthesis of collagen and fibronectin, and on the proliferation of human corneal stromal fibroblasts in vitro, were evaluated. Human corneal stromal fibroblasts in culture were incubated for 48 hours with TGF beta or EGF in the absence of serum. Collagen and fibronectin in the culture media were measured by a collagenase-digestion assay and a competitive ELISA, respectively. The effects of the growth factors on proliferation were assessed by 3H-thymidine incorporation. Collagen synthesis was dose-dependently stimulated by TGF beta; at a concentration of 1 ng/ml of TGF beta, a 120% increase in collagen synthesis was seen over that of controls (p < 0.01). EGF, at a concentration of 10 ng/ml, induced a 40% increase in collagen synthesis over that of controls (p < 0.01). The maximum stimulation by TGF beta was greater than that by EGF (p < 0.05). Fibronectin synthesis was stimulated by TGF beta and EGF in a dose-dependent manner; 230% (p < 0.001) and 210% (p < 0.01) increases in fibronectin synthesis were caused by 10 ng/ml TGF beta and EGF, respectively. TGF beta and EGF dose-dependently stimulated 3H-thymidine incorporation. The maximum increases in 3H-thymidine incorporation reached 180% (p < 0.001) and 190% (p < 0.001) over that in controls, at 10 ng/ml concentrations of TGF beta and EGF, respectively. In conclusion, both TGF beta and EGF are potent stimulants of collagen and fibronectin synthesis and proliferation. Therefore, these two growth factors may be effective alternatives or additional choices for the treatment of corneal ulcer.
评估了转化生长因子-β（TGF-β）和表皮生长因子（EGF）对胶原蛋白和纤连蛋白合成以及体外人角膜基质成纤维细胞增殖的影响。将培养的人角膜基质成纤维细胞在无血清条件下与TGF-β或EGF孵育48小时。分别通过胶原酶消化测定法和竞争性酶联免疫吸附测定法测量培养基中的胶原蛋白和纤连蛋白。通过3H-胸腺嘧啶核苷掺入评估生长因子对增殖的影响。TGF-β以剂量依赖性方式刺激胶原蛋白合成；在TGF-β浓度为1 ng/ml时，胶原蛋白合成比对照增加了120%（p<0.01）。在EGF浓度为10 ng/ml时，胶原蛋白合成比对照增加了40%（p<0.01）。TGF-β的最大刺激作用大于EGF（p<0.05）。TGF-β和EGF以剂量依赖性方式刺激纤连蛋白合成；10 ng/ml的TGF-β和EGF分别使纤连蛋白合成增加230%（p<0.001）和210%（p<0.01）。TGF-β和EGF以剂量依赖性方式刺激3H-胸腺嘧啶核苷掺入。在TGF-β和EGF浓度为10 ng/ml时，3H-胸腺嘧啶核苷掺入的最大增加分别比对照达到180%（p<0.001）和190%（p<0.001）。总之，TGF-β和EGF都是胶原蛋白和纤连蛋白合成以及增殖的有效刺激剂。因此，这两种生长因子可能是治疗角膜溃疡的有效替代方案或额外选择。
