Laboratory of Phytopathology, Federal University of Santa Catarina, 88034-001 Florianópolis, SC, Brazil.
Department of Biochemistry and Molecular Biology, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil.
Carbohydr Polym. 2015 Nov 20;133:384-90. doi: 10.1016/j.carbpol.2015.07.055. Epub 2015 Jul 22.
The present work aimed to evaluate the defense responses induced by chemically sulfated ulvans in Arabidopsis thaliana plants against the phytopathogenic fungi Alternaria brassicicola and Colletotrichum higginsianum. Derivatives with growing sulfate content (from 20.9 to 36.6%) were prepared with SO3-pyridine complex in formamide. NMR and FTIR spectroscopic analyses confirmed the increase of sulfate groups after the chemical sulfation process. The native sulfated polysaccharide (18.9% of sulfate) and its chemically sulfated derivatives similarly reduced the severity of both pathogenic fungi infections. Collectively, our results suggest that ulvans induce resistance against both fungal pathogens independently of its sulfation degree.
本研究旨在评估经化学硫酸酯化后的石莼聚糖在拟南芥植物中对植物病原菌交链格孢菌和炭疽菌的防御反应。采用 SO3-吡啶复合物在甲酰胺中制备了硫酸酯化度不断增加（从 20.9%到 36.6%）的衍生物。NMR 和 FTIR 光谱分析证实了化学硫酸酯化过程后硫酸基团的增加。天然硫酸化多糖（硫酸根含量为 18.9%）及其化学硫酸化衍生物同样减轻了两种病原菌感染的严重程度。综上所述，我们的研究结果表明，石莼聚糖可独立于其硫酸化程度诱导对两种真菌病原体的抗性。
