College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Carbohydr Polym. 2015 Nov 20;133:613-23. doi: 10.1016/j.carbpol.2015.07.044. Epub 2015 Jul 17.
Epimedium polysaccharide-propolis flavone liposome (EPL), a potent immunological pharmaceutical preparation, was investigated for the immunomodulatory activity on Kupffer cells (KCs) in vitro. The results showed that EPL could significantly induce the secretion of chemokines (RANTES and MCP-1), promote the production of nitric oxide and induced nitric oxide synthase, improve the pinocytic and phagocytic activity of KCs, promote the mRNA expression of TNF-α and IL-1β, and enhance the expression of costimulatory molecules (CD11b and CD68) in KCs compared with Epimedium polysaccharide-propolis flavone (EP) at 30-7.5μg/mL. In addition, the abilities of KCs on stimulating lymphocytes proliferation and antigen presenting were significantly enhanced after stimulated with EPL compared with EP. These results suggested that EPL could activate KCs and possessed the stronger immunomodulatory effect, which provided the theoretical basis for further studying the mechanism of EPL on improving the immune response.
朝藿定多糖-蜂胶黄酮脂质体（EPL）是一种有效的免疫性药物制剂，本研究旨在探讨其对体外枯否细胞（KCs）的免疫调节作用。结果表明，EPL 可显著诱导趋化因子（RANTES 和 MCP-1）的分泌，促进一氧化氮的产生和诱导型一氧化氮合酶的表达，提高 KCs 的胞饮和吞噬能力，促进 TNF-α和 IL-1β的 mRNA 表达，并增强 KCs 表面共刺激分子（CD11b 和 CD68）的表达，与 30-7.5μg/mL 的朝藿定多糖-蜂胶黄酮（EP）相比，EPL 具有更强的免疫调节作用。此外，EPL 刺激后 KCs 刺激淋巴细胞增殖和抗原呈递的能力显著增强。这些结果表明，EPL 可激活 KCs，并具有更强的免疫调节作用，为进一步研究 EPL 改善免疫反应的机制提供了理论依据。
