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禁食状态下新生肉仔鸡对葡萄糖酸钙注射的生理适应性指标

Physiological adaptive indicators in fasted neonate broiler chicks in response to calcium gluconate injection.

作者信息

Khosravinia H

机构信息

Department of Animal Science, Faculty of Agriculture, Lorestan University, Khorramabad, 68137-17133, Iran.

出版信息

J Anim Physiol Anim Nutr (Berl). 2016 Jun;100(3):506-12. doi: 10.1111/jpn.12389. Epub 2015 Sep 7.

DOI:10.1111/jpn.12389
PMID:26344414
Abstract

Four hundred and eighty mixed-sex broiler chicks aged 3 h after hatching were allotted according to a completely random design in a 6 × 2 × 2 factorial schedule into two groups of 12 replications of 20 chicks each. The main experimental factors were fasting for 0, 6, 12, 24, 36 and 48 h after chick placement and calcium gluconate (Ca-glu) injection (0 and 0.6 ml). Live body weight (BW) of chicks decreased linearly (Y = 43.36-0.109BW0 h , r(2)  = 0.876) as neonatal fasting extended. Injection of 0.6 ml Ca-glu at 3 h post-hatching did not affect weight loss of chicks. Yolk residuals (YR) utilized linearly (Y = 5.75-0.062YR, r(2)  = 0.956) by 0.062 g/h in neonate fasted chicks up to 48 h, showing no effect of Ca-glu injection. Neonatal fasting periods longer than 12 h increased liver weight (p < 0.05). The mean absolute and proportional (% of BW0 h ) breast and leg weight were reduced linearly as neonatal fasting extended (p < 0.05). Serum glucose concentration increased up to 6 h and then reduced linearly to 150 mg/dl after 48-h fasting. The Ca-glu treatment influenced serum glucose level for a short period up to 6 h of fasting. Serum Ca concentration sharply increased up to threefolds in the birds received Ca-glu injection resulting in acute hypercalcemia, then decreased to the initial level after 24-h feed withdrawal (p < 0.05). The mean serum level for creatinine, uric acid, cholesterol, HDL, albumins and total proteins significantly increased during the fasting periods of 6 to 48 h and significantly elevated in the birds receiving 0.6-ml Ca-glu injection compared with the non-treated chicks (p < 0.05). It was concluded that subcutaneous administration of 0.6 ml Ca-glu in the chick's neck did not suitably support the increased metabolic demands for glucose and calcium in feed-deprived neonate chicks.

摘要

480只刚孵化3小时的混合性别的肉鸡雏鸡,按照完全随机设计,以6×2×2析因试验方案分为两组,每组12个重复,每个重复20只雏鸡。主要实验因素为雏鸡放置后禁食0、6、12、24、36和48小时以及葡萄糖酸钙(Ca - glu)注射(0和0.6毫升)。随着新生雏鸡禁食时间延长,雏鸡的活体体重(BW)呈线性下降(Y = 43.36 - 0.109BW0 h,r(2) = 0.876)。孵化后3小时注射0.6毫升Ca - glu对雏鸡体重减轻没有影响。新生禁食雏鸡在48小时内,卵黄残留量(YR)以0.062克/小时的速度线性减少(Y = 5.75 - 0.062YR,r(2) = 0.956),表明Ca - glu注射没有影响。新生禁食时间超过12小时会增加肝脏重量(p < 0.05)。随着新生禁食时间延长,胸肌和腿部的平均绝对重量和相对重量(占BW0 h的百分比)呈线性下降(p < 0.05)。血清葡萄糖浓度在禁食6小时时升高,然后在禁食48小时后线性降至150毫克/分升。Ca - glu处理在禁食6小时内对血清葡萄糖水平有短期影响。接受Ca - glu注射的鸡血清钙浓度急剧增加至三倍,导致急性高钙血症,然后在禁食24小时后降至初始水平(p < 0.05)。在禁食6至48小时期间,肌酐、尿酸、胆固醇、高密度脂蛋白、白蛋白和总蛋白的平均血清水平显著升高,与未处理的雏鸡相比,接受0.6毫升Ca - glu注射的鸡这些指标显著升高(p < 0.05)。得出的结论是,在雏鸡颈部皮下注射0.

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