Favacho Alexsandra Rodrigues de Mendonça, Andrade Marcelle Novaes, de Oliveira Renata Carvalho, Bonvicino Cibele Rodrigues, D'Andrea Paulo Sergio, de Lemos Elba Regina Sampaio
Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz, (FIOCRUZ), Rio de Janeiro-RJ, Brazil.
Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz, (FIOCRUZ), Rio de Janeiro-RJ, Brazil.
Microbes Infect. 2015 Nov-Dec;17(11-12):889-92. doi: 10.1016/j.micinf.2015.08.014. Epub 2015 Sep 4.
Several rodent-associated Bartonella species cause disease in humans but little is known about their epidemiology in Brazil. The presence of Bartonella spp. in wild rodents captured in two municipalities of the Mato Grosso do Sul state was assessed by polymerase chain reaction (PCR). Fragments of heart tissue from 42 wild rodents were tested using primers targeting the Bartonella 16S-23S intergenic transcribed spacer (ITS) region and citrate synthase gltA gene. The wild rodents were identified based on external and cranial morphology and confirmed at species level by mitochondrial DNA (cytochrome B) sequencing and karyotype. Overall, 42.9% (18/42) of the wild rodents were PCR positive for Bartonella spp.: Callomys callosus (04), Cerradomys maracajuensis (04), Hylaeamus megacephalus (01), Necromys lasiurus (06), Nectomys squamipes (01), Oecomys catherinae (01) and Oxymycterus delator (01). Bartonella vinsonii subsp. arupensis was detected in N. lasiurus (46%) and C. callosus (21%) captured in the two study sites. We reported the first molecular detection of B. vinsonii subsp. arupensis in different species of wild rodents collected in the Brazilian territory. Further studies are needed to examine the role of these mammals in the eco-epidemiology of bartonellosis in Brazil.
几种与啮齿动物相关的巴尔通体菌种可导致人类疾病，但人们对其在巴西的流行病学情况知之甚少。通过聚合酶链反应（PCR）评估了南马托格罗索州两个市捕获的野生啮齿动物中巴尔通体菌种的存在情况。使用针对巴尔通体16S - 23S基因间转录间隔区（ITS）和柠檬酸合酶gltA基因的引物，对42只野生啮齿动物的心脏组织片段进行了检测。根据外部和颅骨形态对野生啮齿动物进行鉴定，并通过线粒体DNA（细胞色素B）测序和核型分析在物种水平上进行确认。总体而言，42.9%（18/42）的野生啮齿动物巴尔通体菌种PCR检测呈阳性：罗氏稻鼠（4只）、马拉卡茹塞拉多鼠（4只）、巨头希拉鼠（1只）、拉氏巢鼠（6只）、鳞足水鼠（1只）、卡氏奥氏鼠（1只）和戴尔托奥氏鼠（1只）。在两个研究地点捕获的拉氏巢鼠（46%）和罗氏稻鼠（21%）中检测到文森巴尔通体阿鲁彭西斯亚种。我们报告了在巴西境内采集的不同野生啮齿动物物种中首次对文森巴尔通体阿鲁彭西斯亚种进行分子检测。需要进一步研究以考察这些哺乳动物在巴西巴尔通体病生态流行病学中的作用。
