Kremr Daniel, Bajerová Petra, Bajer Tomáš, Eisner Aleš, Adam Martin, Ventura Karel
Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic.
J Food Sci Technol. 2015 Sep;52(9):5727-35. doi: 10.1007/s13197-014-1660-8. Epub 2014 Nov 25.
In this study, an optimisation of extraction of sulphur volatile compounds (SVCs) has been performed using Central Composite Design. The conditions of the highest amount of eluated peaks and total peaks area have been treated. Factors such as coating of fiber for SPME (Solid Phase Microextraction), extraction temperature and extraction time have been optimised. The SVCs have shown the optimal extraction using a DVB/CAR/PDMS (divinylbenzene/carboxen/polydimethylsiloxane) fiber at 73 °C during 50 min. Furthermore, a pre-incubation step lasting 20 min at the extraction temperature has been used. In total, 12 samples have been investigated at the mentioned optimal conditions, eight from the Alliaceae and four from the Brassicaceae family. The highest number of SVCs (24) has been identified in the sample of chive. The most frequently identified compound found in 11 of 12 samples has been dimethyl trisulphide.
在本研究中，采用中心复合设计对挥发性含硫化合物（SVCs）的提取进行了优化。对洗脱峰数量和总峰面积最高的条件进行了研究。对固相微萃取（SPME）纤维涂层、萃取温度和萃取时间等因素进行了优化。结果表明，使用DVB/CAR/PDMS（二乙烯基苯/碳分子筛/聚二甲基硅氧烷）纤维在73℃下萃取50分钟时，SVCs的萃取效果最佳。此外，在萃取温度下进行了20分钟的预孵育步骤。总共在上述最佳条件下研究了12个样品，其中8个来自葱属植物，4个来自十字花科植物。韭菜样品中鉴定出的SVCs数量最多（24种）。在12个样品中的11个中最常鉴定出的化合物是二甲基三硫醚。
