猪促卵泡素受体（FSHR）基因第10外显子多态性与产仔数的关系

Relationship between polymorphisms in exon 10 of FSHR gene and litter size in swine.

作者信息

Zhang X D, Zhu H Y, Zhou J, Wang N, Zhou N, Huang L, Wu T, Feng Y F, Ding Y Y, Yin Z J

机构信息

College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.

College of Animal Science and Technology, Anhui Agricultural University, Hefei, China

出版信息

Genet Mol Res. 2015 Jul 27;14(3):8252-61. doi: 10.4238/2015.July.27.13.

DOI:10.4238/2015.July.27.13

PMID:26345751

Abstract

Follicle-stimulating hormone (FSH), a glycoprotein secreted by the anterior pituitary, can regulate ovarian function through the FSH receptor (FSHR). To evaluate the effects of the FSHR gene on reproductive traits in pigs, polymorphisms in exon 10 of the FSHR gene were observed by polymerase chain reaction-single-strand conformation polymorphism, and 3 single nucleotide polymorphisms (C1491T, G1885A, and C1977T) in exon 10 of the porcine FSHR gene, and 3 genotypes (AA, AB, and BB) for C1491T and 2 haplotypes (D and E) for G1885A and C1977T were identified. Further analysis of single nucleotide polymorphism genotypes associated with reproductive traits including total number born (TNB) and number born alive (NBA) was carried out in 3 pig populations including Berkshire, Wannan Black (a Chinese indigenous pig breed), and BW pigs (two-way crossbred pigs produced from Berkshire ♂ and Wannan Black pig ♀). The results showed that the TNB and NBA of Wannan Black pigs with the AB genotype were significantly higher than in AA genotype sows (P < 0.01) in multiparity sows and all parities. The TNB and NBA of Berkshire pigs with the DE genotype were significantly higher than the DD and EE genotype sows (P < 0.01) in gilts, sows and all parities. Overall, TNB and NBA from the 3 identified genotypes was DE > DD > EE. The results showed that polymorphisms in exon 10 of the FSHR gene had a significant effect on litter size traits of Wannan Black and Berkshire pigs. These results can be applied for marker-assisted selection in the 2 swine breeds.

摘要

促卵泡激素（FSH）是一种由垂体前叶分泌的糖蛋白，它可以通过促卵泡激素受体（FSHR）调节卵巢功能。为了评估FSHR基因对猪繁殖性状的影响，通过聚合酶链反应-单链构象多态性观察了FSHR基因第10外显子的多态性，鉴定出猪FSHR基因第10外显子中的3个单核苷酸多态性（C1491T、G1885A和C1977T），以及C1491T的3种基因型（AA、AB和BB）和G1885A与C1977T的2种单倍型（D和E）。在包括伯克夏猪、皖南黑猪（中国地方猪种）和BW猪（由伯克夏公猪♂与皖南黑母猪♀杂交产生的二元杂交猪）在内的3个猪群中，对与繁殖性状（包括总产仔数（TNB）和产活仔数（NBA））相关的单核苷酸多态性基因型进行了进一步分析。结果表明，在经产母猪和所有胎次中，AB基因型皖南黑猪的TNB和NBA显著高于AA基因型母猪（P<0.01）。在后备母猪、经产母猪和所有胎次中，DE基因型伯克夏猪的TNB和NBA显著高于DD和EE基因型母猪（P<0.01）。总体而言，3种已鉴定基因型的TNB和NBA为DE>DD>EE。结果表明，FSHR基因第10外显子的多态性对皖南黑猪和伯克夏猪的窝产仔数性状有显著影响。这些结果可应用于这两个猪品种的标记辅助选择。