利用伯克霍尔德氏菌 Z-90 进行生物淋滤修复重金属污染土壤。
Bioleaching remediation of heavy metal-contaminated soils using Burkholderia sp. Z-90.
机构信息
School of Metallurgy and Environment, Central South University, Changsha 410083, China; Chinese National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, Changsha 410083, China.
School of Metallurgy and Environment, Central South University, Changsha 410083, China.
出版信息
J Hazard Mater. 2016 Jan 15;301:145-52. doi: 10.1016/j.jhazmat.2015.08.047. Epub 2015 Aug 28.
Bioleaching is an environment-friendly and economical technology to remove heavy metals from contaminated soils. In this study, a biosurfactant-producing strain with capacity of alkaline production was isolated from cafeteria sewer sludge and its capability for removing Zn, Pb, Mn, Cd, Cu, and As was investigated. Phylogenetic analysis using 16S rDNA gene sequences confirmed that the strain belonged to Burkholderia sp. and named as Z-90. The biosurfactant was glycolipid confirmed by thin layer chromatography and Fourier-transform infrared spectroscopy. Z-90 broth was then used for bioleaching remediation of heavy metal-contaminated soils. The removal efficiency was 44.0% for Zn, 32.5% for Pb, 52.2% for Mn, 37.7% for Cd, 24.1% for Cu and 31.6% for As, respectively. Mn, Zn and Cd were more easily removed from soil than Cu, Pb and As, which was attributed to the presence of high acid-soluble fraction of Mn, Zn and Cd and high residual fraction of Cu, Pb and As. The heavy metal removal in soils was contributed to the adhesion of heavy metal-contaminated soil minerals with strain Z-90 and the formation of a metal complex with biosurfactant.
生物淋滤是一种从污染土壤中去除重金属的环保且经济的技术。本研究从食堂下水道污泥中分离出了一株具有产碱性能力的产生物表面活性剂的菌株，并研究了其去除 Zn、Pb、Mn、Cd、Cu 和 As 的能力。基于 16S rDNA 基因序列的系统发育分析证实，该菌株属于伯克霍尔德氏菌（Burkholderia），并命名为 Z-90。生物表面活性剂通过薄层层析和傅里叶变换红外光谱确认为糖脂。然后使用 Z-90 发酵液进行重金属污染土壤的生物淋滤修复。Zn、Pb、Mn、Cd、Cu 和 As 的去除效率分别为 44.0%、32.5%、52.2%、37.7%、24.1%和 31.6%。Mn、Zn 和 Cd 比 Cu、Pb 和 As 更容易从土壤中去除，这归因于 Mn、Zn 和 Cd 的酸溶态分数较高，而 Cu、Pb 和 As 的残留态分数较高。土壤中重金属的去除归因于污染土壤矿物与菌株 Z-90 的黏附以及生物表面活性剂与重金属形成的金属络合物。
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