基于红外的分子成像在头颈癌小鼠模型中的应用。
Application of infrared-based molecular imaging to a mouse model with head and neck cancer.
作者信息
Tanaka Nobuaki, Lajud Shayanne A, Ramsey Andrew, Szymanowski Adam R, Ruffner Randall, O'Malley Bert W, Li Daqing
机构信息
Department of Otolaryngology - Head and Neck Surgery, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania.
Department of Otolaryngology - Head and Neck Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan.
出版信息
Head Neck. 2016 Apr;38 Suppl 1:E1351-7. doi: 10.1002/hed.24226. Epub 2015 Sep 8.
BACKGROUND
This study investigated whether near infrared (NIR) or visible fluorescent molecular imaging produced a better representation of a mouse model with head and neck squamous cell carcinoma (HNSCC). Additionally, the study explored whether epidermal growth factor receptor (EGFR)-targeted probes could play an important role in the diagnosis of HNSCC.
METHODS
An orthotopic mouse model of HNSCC labeled with the NIR fluorophore, infrared fluorescent protein (iRFP), was developed and monitored noninvasively in real time. The tumors were further evaluated using tumor-specific EGFR-targeted probes conjugated with an NIR dye (IRDye800), or a visible fluorescent protein.
RESULTS
The iRFP cell line produced better results than cells emitting visible light when studying local, distant, and deep tumors in the mouse model. The EGFR-targeted probe conjugated with IRDye800 accurately detected tumor perimeters.
CONCLUSION
This model has great potential as a unique tool in the study of HNSCC tumor development. © 2015 Wiley Periodicals, Inc. Head Neck 38: E1351-E1357, 2016.
背景
本研究调查了近红外（NIR）或可见光荧光分子成像是否能更好地呈现头颈部鳞状细胞癌（HNSCC）小鼠模型。此外，该研究还探讨了表皮生长因子受体（EGFR）靶向探针在HNSCC诊断中是否能发挥重要作用。
方法
构建了用近红外荧光团红外荧光蛋白（iRFP）标记的HNSCC原位小鼠模型，并对其进行实时无创监测。使用与近红外染料（IRDye800）或可见光荧光蛋白偶联的肿瘤特异性EGFR靶向探针进一步评估肿瘤。
结果
在研究小鼠模型中的局部、远处和深部肿瘤时，iRFP细胞系比发出可见光的细胞产生了更好的结果。与IRDye800偶联的EGFR靶向探针准确检测到了肿瘤边界。
结论
该模型作为研究HNSCC肿瘤发展的独特工具具有巨大潜力。©2015威利期刊公司。《头颈》38：E1351 - E1357，2016年。
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