Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, PR China.
Oncol Rep. 2012 Jun;27(6):1925-31. doi: 10.3892/or.2012.1705. Epub 2012 Feb 29.
In this study, we applied near-infrared fluorescent quantum dots (NIRF-QDs) for non-invasive in vivo and in situ imaging of head and neck squamous cell carcinoma (HNSCC). The U14 squamous cancer cell line with high expression of epidermal growth factor receptor (EGFR) was implanted subcutaneously into the head and neck regions of nude mice to establish HNSCC models. NIRF-QDs with an emission wavelength of 800 nm (NIRF-QD800) were conjugated with EGFR monoclonal antibodies to develop the QD800-EGFR Ab probe. In vivo and in vitro studies demonstrated that the QD800-EGFR Ab probe can specifically bind EGFR expressed on U14 cells. U14 squamous cell carcinoma in the head and neck can be clearly visualized by in vivo imaging after intravenous injection of QD800-EGFR Ab probes. The results suggested that in situ imaging using NIRF-QD-EGFR Ab probes has unique advantages and prospects for the investigation of tumor development, early diagnosis and personalized therapy of HNSCC.
在这项研究中，我们应用近红外荧光量子点（NIRF-QDs）对头颈部鳞状细胞癌（HNSCC）进行非侵入性的体内和原位成像。将高表达表皮生长因子受体（EGFR）的 U14 鳞状癌细胞系皮下植入裸鼠的头颈部，建立 HNSCC 模型。将发射波长为 800nm 的近红外荧光量子点（NIRF-QD800）与 EGFR 单克隆抗体偶联，开发出 QD800-EGFR Ab 探针。体内和体外研究表明，QD800-EGFR Ab 探针可以特异性结合 U14 细胞上表达的 EGFR。静脉注射 QD800-EGFR Ab 探针后，通过体内成像可以清晰地观察到头颈部的 U14 鳞状癌细胞癌。结果表明，使用 NIRF-QD-EGFR Ab 探针进行原位成像对头颈部鳞状细胞癌的发展、早期诊断和个性化治疗的研究具有独特的优势和前景。
