Smith A M
J Immunol. 1976 Feb;116(2):469-74.
Type III pneumococcal polysaccharide (SIII) and bacterial lipopolysaccharide (LPS) were used to evaluate B cell and T cell regulatory functions in BALB/c, SJL/J, and C3H mice of various ages. It was found that the BALB/c and C3H mice could mount high level plaque-forming cell (PFC) responses to SIII at various ages through 110 weeks whereas the levels of the SJL/J PFC responses had begun to decline by the age of 42 weeks through the age of 80 weeks. BALB/c mice were also capable of producing strong PFC responses to LPS at various ages through 110 weeks whereas the comparable SJL/J PFC responses to LPS had declined by 80 weeks of age. By using anti-lymphocyte serum (ALS) and low-dose paralysis to SIII, it was shown that suppressor T cell activity was apparently greater in young BALB/c mice than in older BALB/c mice. It was also found that paralysis to SIII in BALB/c mice was easier to achieve at an early age. SJL/J mice were found to have the necessary B cell activity to respond to SIII through 80 weeks of age and the PFC responses could be greatly enhanced by ALS. Implications of the roles of regulatory T cells in aging are discussed.
使用III型肺炎球菌多糖（SIII）和细菌脂多糖（LPS）来评估不同年龄的BALB/c、SJL/J和C3H小鼠的B细胞和T细胞调节功能。结果发现，BALB/c和C3H小鼠在110周龄前的各个年龄段对SIII都能产生高水平的空斑形成细胞（PFC）反应，而SJL/J小鼠的PFC反应水平在42周龄至80周龄之间就开始下降。BALB/c小鼠在110周龄前的各个年龄段对LPS也能产生强烈的PFC反应，而SJL/J小鼠对LPS的类似PFC反应在80周龄时已经下降。通过使用抗淋巴细胞血清（ALS）和低剂量SIII麻痹，结果表明，年轻BALB/c小鼠的抑制性T细胞活性明显高于老年BALB/c小鼠。还发现，BALB/c小鼠在幼年时更容易实现对SIII的麻痹。发现SJL/J小鼠在80周龄前具有对SIII做出反应所需的B细胞活性，并且PFC反应可以通过ALS大大增强。讨论了调节性T细胞在衰老中的作用的意义。
