花背蟾蜍的完整线粒体基因组。
The complete mitochondrial genome of Bufo raddei.
作者信息
Zhang Wenya, Zhang Xingjie, Guo Rui, Tang Yue, Zhang Yingmei
机构信息
a Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University , Lanzhou , PR China.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3659-60. doi: 10.3109/19401736.2015.1079843. Epub 2015 Sep 10.
The complete mitochondrial genome of Bufo raddei is carried out in the present research using Illumina Hiseq 2500. The mitogenome is 17 602 bp in length and contains 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a D-loop region. The overall base composition of the H-strand is 29.15% for A, 26.09% for C, 15.16% for G, and 29.60% for T. The G + C content is 41.25%. Phylogenetic analyses of B. raddei and other 12 amphibian were carried out using Bayesian phylogenetic methods. The sequences of B. raddei were clustered in genus Bufo.
本研究利用Illumina Hiseq 2500对花背蟾蜍的完整线粒体基因组进行了测序。该线粒体基因组长度为17602 bp，包含13个蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因和一个控制区。重链的总体碱基组成为：A占29.15%，C占26.09%，G占15.16%，T占29.60%。G+C含量为41.25%。采用贝叶斯系统发育方法对花背蟾蜍和其他12种两栖动物进行了系统发育分析。花背蟾蜍的序列聚类在蟾蜍属中。
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