Bhattacharyya M, Chaudhuri U, Poddar R K
Department of Biophysics, Molecular Biology and Genetics, University of Calcutta, India.
Biochem Int. 1989 Dec;19(6):1363-72.
The binding of chlorpromazine (CPZ), an widely used tranquilizer, with hemoglobin (Hb) at pH 6.5 has been investigated by means of spectrophotometry, circular dichroism (CD), and equilibrium dialysis. In CD spectra Hb treated with CPZ exhibited a blue shift of 5 nm in the visible wavelength range. The positively cooperative nature of binding was revealed by equilibrium dialysis experiments. The basic parameters, namely, the cooperative binding constant (K), the degree of cooperativity (q), and the number of amino acids (n) occupied by one CPZ molecule were evaluated from spectrophotometric and dialysis experiments and found to be sensitive to NaCl concentration, suggesting the electrostatic nature of the binding process.
通过分光光度法、圆二色性（CD）和平衡透析法研究了广泛使用的镇静剂氯丙嗪（CPZ）在pH 6.5时与血红蛋白（Hb）的结合情况。在CD光谱中，用CPZ处理的Hb在可见波长范围内出现了5nm的蓝移。平衡透析实验揭示了结合的正协同性质。通过分光光度法和透析实验评估了基本参数，即协同结合常数（K）、协同度（q）和一个CPZ分子占据的氨基酸数（n），发现这些参数对NaCl浓度敏感，表明结合过程具有静电性质。
