Bhattacharyya M, Chaudhuri U, Poddar R K
Department of Biophysics, Molecular Biology & Genetics, University of Calcutta, India.
Indian J Biochem Biophys. 1990 Aug;27(4):240-4.
Binding of chlorpromazine (CPZ), a widely used antidepressant tranquilizer, with hemoglobin has been studied by equilibrium dialysis method. r/Cf versus r plot was typically concave downwards revealing the positive cooperative nature of binding. Binding parameters, namely the affinity constant (K) and the degree of cooperativity (nH) were determined from the Hill plot. Oxygen was found to be released gradually from hemoglobin with gradual addition of CPZ, the extent of oxygen release depending on the stoichiometric ratio of CPZ: hemoglobin (D/P).
已通过平衡透析法研究了广泛使用的抗抑郁镇静剂氯丙嗪（CPZ）与血红蛋白的结合。r/Cf对r图通常向下凹，表明结合具有正协同性质。结合参数，即亲和常数（K）和协同度（nH），由希尔图确定。发现随着CPZ的逐渐加入，氧气从血红蛋白中逐渐释放，氧气释放的程度取决于CPZ与血红蛋白的化学计量比（D/P）。
