Cloning of a thermostable alpha-amylase gene from Thermomonospora curvata and its expression in Streptomyces lividans.
作者信息
Petrícek M, Stajner K, Tichý P
机构信息
Department of General Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
出版信息
J Gen Microbiol. 1989 Dec;135(12):3303-9. doi: 10.1099/00221287-135-12-3303.
PMID:2636261
Abstract
The gene from Thermomonospora curvata CCM 3312 coding for thermostable alpha-amylase (tam) has been cloned in Streptomyces lividans TK 24 and localized to a 2.6 kb HindIII-BamHI fragment of DNA. The data presented here show that the tam gene is expressed at a high level in S. lividans and that the protein is efficiently excreted.
摘要
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