从嗜热放线菌弯曲热单孢菌中分离依赖DNA的RNA聚合酶。
Isolation of DNA-dependent RNA polymerase from the thermophilic actinomycete Thermomonospora curvata.
作者信息
Krausová M, Klégr M, Spízek J
机构信息
Institute of Microbiology, Academy of Sciences of Czech Republic, Prague.
出版信息
Folia Microbiol (Praha). 1994;39(1):7-12. doi: 10.1007/BF02814521.
PMID:8181783
Abstract
DNA-Dependent RNA polymerase (EC 2.7.7.6) was isolated from Thermomonospora curvata. The purification steps included precipitation with Polymin P, elution of the precipitate with 0.3 mol/L KCl, precipitation with ammonium sulfate, affinity chromatography on heparin-agarose and molecular filtration on Biogel A 1.5 m.
摘要
依赖DNA的RNA聚合酶（EC 2.7.7.6）从弯曲嗜热放线菌中分离得到。纯化步骤包括用聚凝胺P沉淀、用0.3mol/L氯化钾洗脱沉淀、硫酸铵沉淀、肝素-琼脂糖亲和层析以及在Biogel A 1.5m上进行分子过滤。
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