Whitehouse Chris A, Chase Kitty, Embers Monica E, Kulesh David A, Ladner Jason T, Palacios Gustavo F, Minogue Timothy D
Molecular and Translational Sciences Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA.
Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA.
J Med Primatol. 2015 Dec;44(6):364-72. doi: 10.1111/jmp.12196. Epub 2015 Sep 14.
Moraxella macacae is a recently described bacterial pathogen that causes epistaxis or so-called bloody nose syndrome in captive macaques. The aim of this study was to develop specific molecular diagnostic assays for M. macacae and to determine their performance characteristics.
We developed six real-time PCR assays on the Roche LightCycler. The accuracy, precision, selectivity, and limit of detection (LOD) were determined for each assay, in addition to further validation by testing nasal swabs from macaques presenting with epistaxis at the Tulane National Primate Research Center.
All assays exhibited 100% specificity and were highly sensitive with an LOD of 10 fg for chromosomal assays and 1 fg for the plasmid assay. Testing of nasal swabs from 10 symptomatic macaques confirmed the presence of M. macacae in these animals.
We developed several accurate, sensitive, and species-specific real-time PCR assays for the detection of M. macacae in captive macaques.
猕猴莫拉菌是一种最近被描述的细菌病原体,可导致圈养猕猴出现鼻出血或所谓的流鼻血综合征。本研究的目的是开发针对猕猴莫拉菌的特异性分子诊断检测方法,并确定其性能特征。
我们在罗氏LightCycler上开发了六种实时荧光定量PCR检测方法。除了通过检测杜兰国家灵长类动物研究中心出现鼻出血的猕猴的鼻拭子进行进一步验证外,还确定了每种检测方法的准确性、精密度、选择性和检测限(LOD)。
所有检测方法均表现出100%的特异性,对染色体检测方法高度敏感,检测限为10 fg,对质粒检测方法为1 fg。对10只出现症状的猕猴的鼻拭子检测证实了这些动物中存在猕猴莫拉菌。
我们开发了几种准确、灵敏且物种特异性的实时荧光定量PCR检测方法,用于检测圈养猕猴中的猕猴莫拉菌。