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静脉注射人骨髓间充质基质细胞可调节放射性脊髓病大鼠模型中的氧化还原状态。

Intravenous Injections of Human Mesenchymal Stromal Cells Modulated the Redox State in a Rat Model of Radiation Myelopathy.

作者信息

Zhang Jing, Li Lian-Bing, Qiu Zhu, Ren Hong-Bo, Wu Jia-Yan, Wang Tao, Bao Zhong-Hui, Yang Ji-Fan, Zheng Ke, Li Shao-Lin, Wei Li, You Hua

机构信息

The First Affiliated Hospital of Liaoning Medical University, No. 2 Renmin Street, Guta District, Jinzhou, Liaoning 121000, China.

Key Laboratory of Birth Defects and Reproductive Health of The National Health and Family Planning Commission, Chongqing Population and The Family Planning Science and Technology Research Institute, Chongqing 400020, China.

出版信息

Evid Based Complement Alternat Med. 2015;2015:432369. doi: 10.1155/2015/432369. Epub 2015 Aug 23.

DOI:10.1155/2015/432369
PMID:26366180
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4561091/
Abstract

The main aim of the present study was to assess the antioxidative effects of human umbilical cord-derived mesenchymal stromal cells (UC-MSCs) in a rat model of radiation myelopathy. UC-MSCs were isolated from Wharton's jelly (WJ) of umbilical cords. An irradiated cervical spinal cord rat model (C2-T2 segment) was generated using a (60)Co irradiator to deliver 30 Gy of radiation. UC-MSCs were injected through the tail vein at 90 days, 97 days, 104 days, and 111 days after-irradiation. Histological damage was examined by cresyl violet/Nissl staining. The activities of two antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPX) in the spinal cord were measured by the biomedical assay. In addition, the levels of vascular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2) in the spinal cord were determined by ELISA methods. Multiple injections of UC-MSCs through the tail vein ameliorated neuronal damage in the spinal cord, increased the activities of the antioxidant enzymes CAT and GPX, and increased the levels of VEGF and Ang-2 in the spinal cord. Our results suggest that multiple injections of UC-MSCs via the tail vein in the rat model of radiation myelopathy could significantly improve the antioxidative microenvironment in vivo.

摘要

本研究的主要目的是评估人脐带间充质基质细胞（UC-MSCs）在放射性脊髓病大鼠模型中的抗氧化作用。UC-MSCs从脐带华通氏胶（WJ）中分离。使用（60）Co辐照器对大鼠C2-T2节段颈脊髓进行照射，给予30 Gy辐射，建立放射性颈脊髓损伤大鼠模型。在照射后90天、97天、104天和111天经尾静脉注射UC-MSCs。通过甲酚紫/尼氏染色检查组织学损伤。采用生物医学检测法测定脊髓中两种抗氧化酶过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）的活性。此外，采用酶联免疫吸附测定（ELISA）法测定脊髓中血管内皮生长因子（VEGF）和血管生成素-2（Ang-2）的水平。经尾静脉多次注射UC-MSCs可改善脊髓神经元损伤，提高抗氧化酶CAT和GPX的活性，并提高脊髓中VEGF和Ang-2的水平。我们的结果表明，在放射性脊髓病大鼠模型中经尾静脉多次注射UC-MSCs可显著改善体内抗氧化微环境。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d79/4561091/11622b7bfc2d/ECAM2015-432369.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d79/4561091/316bbbe0fcb3/ECAM2015-432369.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d79/4561091/137c810e916a/ECAM2015-432369.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d79/4561091/82b964a5d077/ECAM2015-432369.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d79/4561091/b5c61e662c23/ECAM2015-432369.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d79/4561091/11622b7bfc2d/ECAM2015-432369.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d79/4561091/316bbbe0fcb3/ECAM2015-432369.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d79/4561091/137c810e916a/ECAM2015-432369.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d79/4561091/82b964a5d077/ECAM2015-432369.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d79/4561091/b5c61e662c23/ECAM2015-432369.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d79/4561091/11622b7bfc2d/ECAM2015-432369.005.jpg

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Radiation-induced endothelial cell loss and reduction of the relative magnitude of the blood flow in the rat spinal cord.辐射诱导大鼠脊髓内皮细胞损失及血流量相对幅度降低。
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