Yuan Wei, Veenhuis Marten, van der Klei Ida J
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, the Netherlands.
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, the Netherlands.
Biochim Biophys Acta. 2016 May;1863(5):902-10. doi: 10.1016/j.bbamcr.2015.09.008. Epub 2015 Sep 11.
This contribution describes the phenotypic differences of yeast peroxisome-deficient mutants (pex mutants). In some cases different phenotypes were reported for yeast mutants deleted in the same PEX gene. These differences are most likely related to the marker proteins and methods used to detect peroxisomal remnants. This is especially evident for pex3 and pex19 mutants, where the localization of receptor docking proteins (Pex13, Pex14) resulted in the identification of peroxisomal membrane remnants, which do not contain other peroxisomal membrane proteins, such as the ring proteins Pex2, Pex10 and Pex12. These structures in pex3 and pex19 cells are the template for peroxisome formation upon introduction of the missing gene. Taken together, these data suggest that in all yeast pex mutants analyzed so far peroxisomes are not formed de novo but use membrane remnant structures as a template for peroxisome formation upon reintroduction of the missing gene. The relevance of this model for peroxisomal membrane protein and lipid sorting to peroxisomes is discussed.
本论文描述了酵母过氧化物酶体缺陷型突变体（pex突变体）的表型差异。在某些情况下，报道了在同一PEX基因中缺失的酵母突变体具有不同的表型。这些差异很可能与用于检测过氧化物酶体残余物的标记蛋白和方法有关。这在pex3和pex19突变体中尤为明显，其中受体对接蛋白（Pex13、Pex14）的定位导致了过氧化物酶体膜残余物的鉴定，这些残余物不包含其他过氧化物酶体膜蛋白，如环状蛋白Pex2、Pex10和Pex12。在pex3和pex19细胞中的这些结构是在引入缺失基因后过氧化物酶体形成的模板。综上所述，这些数据表明，在迄今为止分析的所有酵母pex突变体中，过氧化物酶体不是从头形成的，而是在重新引入缺失基因后，利用膜残余结构作为过氧化物酶体形成的模板。本文讨论了该模型对于过氧化物酶体膜蛋白和脂质分选至过氧化物酶体的相关性。
