Melton James Theodore, Lopez-Bautista Juan Manuel
a Department of Biological Sciences , The University of Alabama , Tuscaloosa , AL , USA.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3817-9. doi: 10.3109/19401736.2015.1082095. Epub 2015 Sep 15.
We present the 61 614 bp circular-mapping mitochondrial genome of Ulva fasciata. Fifty-eight genes were identified including 29 protein-coding genes, 27 transfer RNA (tRNA) genes, and two ribosomal RNA (rRNA) genes. Four ORFs from the Ulva sp. UNA00071828 mitogenome were conserved compared to the mitogenome of Ulva fasciata. The ∼10 000 bp size difference was mostly due to fewer introns found in U. fasciata mtDNA (4) as compared with the Ulva sp. UNA00071828 mtDNA (10). Introns were annotated in cox1 (3) and nad3 (1). The AT content was similar to Ulva sp. UNA00071828 mtDNA at 67.5%. A phylogenomic anaylsis of 28 chlorophytes and 17 protein-coding genes (14 115 bp alignment) showed that U. fasciata clusters closely with other members of the Ulvophyceae (Ulva sp. UNA00071828, Pseudendoclonium akinetum, and Oltmannsiellopsis viridis). This analysis did not recover a monophyletic Ulvophyceae, however, the Trebouxiophyceae and Chlorophyceae were supported as monophyletic groups.
我们展示了石莼的61614 bp环状线粒体基因组。共鉴定出58个基因,包括29个蛋白质编码基因、27个转运RNA(tRNA)基因和2个核糖体RNA(rRNA)基因。与石莼的线粒体基因组相比,石莼属UNA00071828线粒体基因组中的4个开放阅读框(ORF)是保守的。约10000 bp的大小差异主要是由于石莼线粒体DNA(mtDNA)中内含子较少(4个),而石莼属UNA00071828 mtDNA中有10个内含子。内含子在细胞色素氧化酶亚基I(cox1)(3个)和NADH脱氢酶亚基3(nad3)(1个)中被注释。其AT含量与石莼属UNA00071828 mtDNA相似,为67.5%。对28种绿藻和17个蛋白质编码基因(14115 bp比对)进行的系统基因组分析表明,石莼与石莼科的其他成员(石莼属UNA00071828、假柄绿藻和绿色奥氏藻)紧密聚类。然而,该分析并未得出石莼科为单系类群的结论,不过,支持了小球藻科和绿藻纲为单系类群。