Melton James T, Lopez-Bautista Juan M
a Department of Biological Sciences , the University of Alabama , Tuscaloosa , AL , USA.
Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Jan;28(1):93-95. doi: 10.3109/19401736.2015.1110814. Epub 2015 Dec 28.
We present the 96 005 bp circular chloroplast genome (cpDNA) of Ulva fasciata. This cpDNA was ∼4000 bp smaller than the cpDNA of Ulva sp. UNA00071828; however, this cpDNA was AT rich (75.1%) similar to Ulva sp. The U. fasciata cpDNA was also similar in gene content (101 identified genes) compared to Ulva sp., which included 71 protein-coding genes, 3 ribosomal RNAs (rRNAs) and 27 transfer RNAs (tRNAs). Only one tRNA, trnN(AUU), that was present in Ulva sp. was absent in U. fasciata. Five introns were present in the following genes of U. fasciata: petB (2), psbD (1), psaB (1) and rrl (1). Ulva sp. lacked introns in psbD and psaB, and introns present in atpA and psbB in Ulva sp. were absent in the homologous genes of U. fasciata. A gene arrangement comparison of both Ulva species showed that a ∼27 000 bp segment of DNA consisting of genes psbB to trnT(UGU) was inverted. Furthermore, a phylogenetic analysis of 1135 bp of the rbcL gene confirmed that this cpDNA and the previously published mitochondrial genome from this sample were indeed from U. fasciata.
我们展示了石莼的96005 bp环状叶绿体基因组（cpDNA）。该cpDNA比石莼属UNA00071828的cpDNA小约4000 bp；然而，该cpDNA富含AT（75.1%），与石莼属相似。与石莼属相比，石莼的cpDNA在基因含量上也相似（鉴定出101个基因），其中包括71个蛋白质编码基因、3个核糖体RNA（rRNA）和27个转移RNA（tRNA）。石莼中仅缺少石莼属中存在的一个tRNA，即trnN(AUU)。石莼的以下基因中存在5个内含子：petB（2个）、psbD（1个）、psaB（1个）和rrl（1个）。石莼属在psbD和psaB中缺乏内含子，并且石莼属中atpA和psbB中存在的内含子在石莼的同源基因中不存在。两种石莼的基因排列比较表明，由基因psbB至trnT(UGU)组成的约27000 bp的DNA片段发生了倒位。此外，对1135 bp的rbcL基因进行的系统发育分析证实，该cpDNA和该样本先前发表的线粒体基因组确实来自石莼。
