Krstajić Nikola, Poland Simon, Levitt James, Walker Richard, Erdogan Ahmet, Ameer-Beg Simon, Henderson Robert K
Opt Lett. 2015 Sep 15;40(18):4305-8. doi: 10.1364/OL.40.004305.
We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) events from a 32×32 complementary metal oxide semiconductor (CMOS) single photon avalanche detector (SPAD) array (Megaframe) to the computer memory. Custom firmware was written to transmit event codes from 1024-TCSPC-enabled pixels for fast transfer of TCSPC events. Our 1024-channel TCSPC system is capable of acquiring up to 0.5×10(9) TCSPC events per second with 16 histogram bins spanning a 14 ns width. Other options include 320×10(6) TCSPC events per second with 256 histogram bins spanning either a 14 or 56 ns time window. We present a wide-field fluorescence microscopy setup demonstrating fast fluorescence lifetime data acquisition. To the best of our knowledge, this is the fastest direct TCSPC transfer from a single photon counting device to the computer to date.
我们展示了一种数字架构,用于将与时间相关的单光子计数(TCSPC)事件从32×32互补金属氧化物半导体(CMOS)单光子雪崩探测器(SPAD)阵列(巨型帧)快速采集到计算机内存中。编写了定制固件,以从1024个支持TCSPC的像素传输事件代码,以便快速传输TCSPC事件。我们的1024通道TCSPC系统能够每秒采集高达0.5×10⁹个TCSPC事件,具有16个直方图 bins,跨越14 ns宽度。其他选项包括每秒320×10⁶个TCSPC事件,具有256个直方图 bins,跨越14或56 ns时间窗口。我们展示了一种宽场荧光显微镜设置,证明了快速荧光寿命数据采集。据我们所知,这是迄今为止从单光子计数设备到计算机的最快直接TCSPC传输。