用于基于CMOS单光子传感器的多焦点多光子显微镜的先进荧光寿命成像算法。

Advanced fluorescence lifetime imaging algorithms for CMOS single-photon sensor based multi-focal multi-photon microscopy.

作者信息

Li D D-U, Poland S, Coelho S, Tyndall D, Zhang W, Richardson J, Henderson R K, Ameer-Beg S M

出版信息

Annu Int Conf IEEE Eng Med Biol Soc. 2013;2013:3036-9. doi: 10.1109/EMBC.2013.6610180.

DOI:10.1109/EMBC.2013.6610180

Abstract

We have developed a fast hardware friendly bi-exponential fluorescence lifetime algorithm suitable for 2D CMOS single-photon avalanche diode (SPAD) arrays. The performance of the proposed algorithm against other techniques is demonstrated on the data from a plant specimen (Convallaria) by using 0.13µm CMOS SPAD arrays mounted on a multi-beam multi-photon microscopy system.

摘要

我们已经开发出一种快速且硬件友好的双指数荧光寿命算法，适用于二维互补金属氧化物半导体单光子雪崩二极管（SPAD）阵列。通过使用安装在多光束多光子显微镜系统上的0.13μm CMOS SPAD阵列，在植物标本（铃兰）的数据上展示了所提出算法相对于其他技术的性能。