Lee Cheonghoon, Marion Jason W, Cheung Melissa, Lee Chang Soo, Lee Jiyoung
Division of Environmental Health Sciences, College of Public Health, Ohio State University, Columbus, OH 43210, USA.
Department of Food Sciences & Technology, Ohio State University, Columbus, OH 43210, USA.
Int J Environ Res Public Health. 2015 Sep 11;12(9):11466-85. doi: 10.3390/ijerph120911466.
Lake Erie beaches exhibit impaired water quality due to fecal contamination and cyanobacterial blooms, though few studies address potential relationships between these two public health hazards. Using quantitative polymerase chain reaction (qPCR), Microcystis aeruginosa was monitored in conjunction with a human-associated fecal marker (Bacteroides fragilis group; g-Bfra), microcystin, and water quality parameters at two beaches to evaluate their potential associations. During the summer of 2010, water samples were collected 32 times from both Euclid and Villa Angela beaches. The phycocyanin intergenic spacer (PC-IGS) and the microcystin-producing (mcyA) gene in M. aeruginosa were quantified with qPCR. PC-IGS and mcyA were detected in 50.0% and 39.1% of samples, respectively, and showed increased occurrences after mid-August. Correlation and regression analyses showed that water temperature was negatively correlated with M. aeruginosa markers and microcystin. The densities of mcyA and the g-Bfra were predicted by nitrate, implicating fecal contamination as contributing to the growth of M. aeruginosa by nitrate loading. Microcystin was correlated with mcyA (r = 0.413, p < 0.01), suggesting toxin-producing M. aeruginosa populations may significantly contribute to microcystin production. Additionally, microcystin was correlated with total phosphorus (r = 0.628, p < 0.001), which was higher at Euclid (p < 0.05), possibly contributing to higher microcystin concentrations at Euclid.
伊利湖海滩因粪便污染和蓝藻水华而水质受损，不过很少有研究探讨这两种公共卫生危害之间的潜在关系。利用定量聚合酶链反应（qPCR），在两个海滩对铜绿微囊藻与一种人类相关粪便标志物（脆弱拟杆菌群；g-Bfra）、微囊藻毒素及水质参数进行了监测，以评估它们之间的潜在关联。2010年夏季，从欧几里得海滩和安吉拉别墅海滩共采集了32次水样。用qPCR对铜绿微囊藻中的藻蓝蛋白基因间隔区（PC-IGS）和产微囊藻毒素（mcyA）基因进行了定量分析。分别在50.0%和39.1%的样本中检测到了PC-IGS和mcyA，且在8月中旬之后出现频率增加。相关性和回归分析表明，水温与铜绿微囊藻标志物及微囊藻毒素呈负相关。mcyA和g-Bfra的密度可由硝酸盐预测，这意味着粪便污染通过硝酸盐负荷促进了铜绿微囊藻的生长。微囊藻毒素与mcyA相关（r = 0.413，p < 0.01），表明产毒素铜绿微囊藻种群可能对微囊藻毒素的产生有显著贡献。此外，微囊藻毒素与总磷相关（r = 0.628，p < 0.001），欧几里得海滩的总磷含量更高（p < 0.05），这可能是导致欧几里得海滩微囊藻毒素浓度更高的原因。
