非标准氨基酸的掺入。
Incorporation of Non-Canonical Amino Acids.
作者信息
Leisle Lilia, Valiyaveetil Francis, Mehl Ryan A, Ahern Christopher A
机构信息
Department of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Road, 52246, Iowa City, IA, USA.
Department of Physiology and Pharmacology, Oregon Health and Sciences University, 97239, Portland, OR, USA.
出版信息
Adv Exp Med Biol. 2015;869:119-51. doi: 10.1007/978-1-4939-2845-3_7.
Abstract
In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical production of semisynthetic proteins have been used successfully for ion channel and receptor studies. The level of difficulty for the application of each approach ranges from trivial to technically demanding, yet all have untapped potential in their application to membrane proteins.
摘要
在本章中，我们将讨论三种用于重新编程膜蛋白中特定氨基酸化学组成的方法的优势、注意事项和技术考量。非洲爪蟾卵母细胞中的体内无义抑制、进化出的正交tRNA和氨酰-tRNA合成酶对以及用于半合成蛋白质生化生产的蛋白质连接已成功用于离子通道和受体研究。每种方法的应用难度从简单到技术要求高不等，但在应用于膜蛋白方面都有尚未开发的潜力。
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