Department of Chemistry, Virginia Commonwealth University (VCU), 1001 West Main Street, P.O. Box 842006, Richmond, Virginia 23284, USA.
Org Biomol Chem. 2018 Feb 14;16(7):1073-1078. doi: 10.1039/c7ob02931d.
The ability to incorporate non-canonical amino acids (ncAA) using translation offers researchers the ability to extend the functionality of proteins and peptides for many applications including synthetic biology, biophysical and structural studies, and discovery of novel ligands. Here we describe the high promiscuity of an editing-deficient valine-tRNA synthetase (ValRS T222P). Using this enzyme, we demonstrate ribosomal translation of 11 ncAAs including those with novel side chains, α,α-disubstitutions, and cyclic β-amino acids.
利用翻译将非规范氨基酸(ncAA)整合到蛋白质中,为研究人员提供了扩展蛋白质和肽功能的能力,可应用于包括合成生物学、生物物理和结构研究以及新型配体发现等多个领域。在这里,我们描述了一种具有高度混杂性的丙氨酸-tRNA 合成酶(ValRS T222P)。使用这种酶,我们展示了核糖体对 11 种 ncAA 的翻译,包括具有新型侧链、α,α-二取代和环状β-氨基酸的 ncAA。