葡萄籽原花青素提取物抑制牙龈卟啉单胞菌的脂多糖
[Grape seed proanthocyanidin extracts inhibit lipopolysaccharide of Porphyromonas gingivalis].
作者信息
Ci Xiang-ke, Chen Li-pei, Ou Xiao-yan
机构信息
Department of Preventive Dentistry, Affiliated Stomatological Hospital of Nanchang University. Nanchang 330006, Jiangxi Province, China.E-mail:
出版信息
Shanghai Kou Qiang Yi Xue. 2015 Aug;24(4):433-6.
PURPOSE
To study the inhibitory effect of grape seed proanthocyanidin extracts on 1ipopolysacharides of Porphyromonas gingivalis.
METHODS
Grape seed proanthocyanidin extracts were double-diluted to different concentrations by two-fold dilution. The effect on Porphyromonas gingivalis was measured by minimal inhibitory concentration (MIC). The inhibition effect of grape seed proanthocyanidin extracts on lipopolysaccharide was measured by limulus assay with 6 concentrations below the minimal inhibitory concentration. SPSS 17.0 software package was used for statistical analysis.
RESULTS
The value of MIC of grape seed proanthocyanidin extracts was 0.8 mg/mL to the strains of the experimental bacteria. The inhibitory effect on lipopolysaccharide enhanced with the increasing of the concentration of grape seed proanthocyanidin extracts within the scope of 0.05 to 0.4 mg/mL (P<0.05,0.01).
CONCLUSIONS
Grape seed proanthocyanidin extracts have inhibitory effect on lipopolysaccharide of Porphyromonas gingivalis.
目的
研究葡萄籽原花青素提取物对牙龈卟啉单胞菌脂多糖的抑制作用。
方法
采用二倍稀释法将葡萄籽原花青素提取物稀释成不同浓度，用最低抑菌浓度（MIC）法测定其对牙龈卟啉单胞菌的作用，采用鲎试剂法在低于最低抑菌浓度的6个浓度下测定葡萄籽原花青素提取物对脂多糖的抑制作用，应用SPSS 17.0软件包进行统计学分析。
结果
葡萄籽原花青素提取物对实验菌株的MIC值为0.8mg/mL，在0.05～0.4mg/mL范围内，其对脂多糖的抑制作用随葡萄籽原花青素提取物浓度的增加而增强（P<0.05，0.01）。
结论
葡萄籽原花青素提取物对牙龈卟啉单胞菌脂多糖有抑制作用。
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