Pyatin V F, Tatarnikov V S
Department of Normal Physiology of the Samara State Medical University, Ministry of Health of the Russian Federation, Samara, Russia.
Bull Exp Biol Med. 2015 Aug;159(4):420-3. doi: 10.1007/s10517-015-2980-z. Epub 2015 Sep 21.
In narcotized (40 mg/kg sodium ethaminal intraperitoneally) spontaneously breathing albino rats, experimental short-term hypoxia induced BP drop and increased phrenic nerve firing rate. Unilateral microinjection of a selective NMDA receptor blocker ketamine hydrochloride (50 nl; 4 mM) into A5 area did not affect BP and phrenic nerve fi ring rate. However, against the background of preliminary NMDA receptor blockage, hypoxia more markedly stimulated rhythmic activity of the respiratory center and hypotensive response. Unilateral microinjection of a selective blocker of non-NMDA receptors GAMS (50 nl; 4 mM) into A5 area did not change initial BP and phrenic nerve fi ring rate. The studied parameters in rats exposed to hypoxia after blockage of non-NMDA receptors in A5 area did not differ from the control levels. Thus, activity of the respiratory center and BP in rats during hypoxia is regulated by the structures in A5 area and NMDA glutamate receptors.
在经腹腔注射40毫克/千克戊巴比妥钠麻醉且自主呼吸的白化大鼠中，实验性短期缺氧导致血压下降并增加膈神经放电频率。向A5区域单侧微量注射选择性NMDA受体阻滞剂盐酸氯胺酮（50纳升；4毫摩尔）不影响血压和膈神经放电频率。然而，在预先阻断NMDA受体的背景下，缺氧更明显地刺激了呼吸中枢的节律性活动和降压反应。向A5区域单侧微量注射非NMDA受体选择性阻滞剂GAMS（50纳升；4毫摩尔）不改变初始血压和膈神经放电频率。在A5区域阻断非NMDA受体后暴露于缺氧环境的大鼠中，所研究的参数与对照水平无差异。因此，缺氧期间大鼠呼吸中枢的活动和血压受A5区域结构和NMDA谷氨酸受体调节。
