牛磺酸在7,12-二甲基苯并[a]蒽诱导的实验性乳腺癌发生过程中调节线粒体功能。
Taurine Regulates Mitochondrial Function During 7,12-Dimethyl Benz[a]anthracene Induced Experimental Mammary Carcinogenesis.
作者信息
Vanitha Manickam Kalappan, Priya Kalpana Deepa, Baskaran Kuppusamy, Periyasamy Kuppusamy, Saravanan Dhravidamani, Venkateswari Ramachandran, Mani Balasundaram Revathi, Ilakkia Aruldass, Selvaraj Sundaramoorthy, Menaka Rajendran, Geetha Mahendran, Rashanthy Nadarajah, Anandakumar Pandi, Sakthisekaran Dhanapal
机构信息
Department of Medical Biochemistry, University of Madras, Taramani Campus, Chennai, India.
Department of Biomedical Sciences, College of Health Sciences, Arsi University, Asella, Ethiopia.
出版信息
J Pharmacopuncture. 2015 Sep;18(3):68-74. doi: 10.3831/KPI.2015.18.027.
OBJECTIVES
The present study was undertaken to determine the modulatory effect of taurine on the liver mitochondrial enzyme system with reference to mitochondrial lipid peroxidation (LPO), antioxidants, major tricarboxylic acid cycle enzymes, and electron transport chain enzymes during 7,12-dimethyl benz[a]anthracene (DMBA) induced breast cancer in Sprague-Dawley rats.
METHODS
Animals in which breast cancer had been induced by using DMBA (25 mg/kg body weight) showed an increase in mitochondrial LPO together with decreases in enzymic antioxidants (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST)), non-enzymic antioxidants (reduced glutathione (GSH), vitamin C, and vitamin E), in citric acid cycle enzymes (isocitrate dehydrogenase (ICDH), alpha ketoglutarate dehydrogenase (alpha KDH), succinate dehydrogenase (SDH) and malate dehydrogenase (MDH)), and in electron transport chain (ETC) complexes.
RESULTS
Taurine (100 mg/kg body weight) treatment decreased liver mitochondrial LPO and augmented the activities/levels of enzymic, and non-enzymic antioxidants, tricarboxylic acid cycle enzymes and ETC complexes.
CONCLUSION
The results of our present study demonstrated the chemotherapeutic efficacy of taurine treatment for DMBA-induced breast carcinomas.
目的
本研究旨在确定牛磺酸对7,12-二甲基苯并[a]蒽（DMBA）诱导的Sprague-Dawley大鼠乳腺癌模型肝脏线粒体酶系统的调节作用，该作用涉及线粒体脂质过氧化（LPO）、抗氧化剂、主要三羧酸循环酶和电子传递链酶。
方法
用DMBA（25mg/kg体重）诱导乳腺癌的动物，其线粒体LPO增加，同时酶促抗氧化剂（超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）和谷胱甘肽-S-转移酶（GST））、非酶促抗氧化剂（还原型谷胱甘肽（GSH）、维生素C和维生素E）、柠檬酸循环酶（异柠檬酸脱氢酶（ICDH）、α-酮戊二酸脱氢酶（α-KDH）、琥珀酸脱氢酶（SDH）和苹果酸脱氢酶（MDH））以及电子传递链（ETC）复合物的水平降低。
结果
牛磺酸（100mg/kg体重）处理可降低肝脏线粒体LPO，并提高酶促和非酶促抗氧化剂、三羧酸循环酶和ETC复合物的活性/水平。
结论
我们目前的研究结果证明了牛磺酸治疗DMBA诱导的乳腺癌的化疗效果。
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