不同皮肤癌亚型中假定的癌症干细胞标志物CD44和醛脱氢酶1A1（ALDH1A1）的比较表达分析。

Comparative expression analysis of putative cancer stem cell markers CD44 and ALDH1A1 in various skin cancer subtypes.

作者信息

Erfani Elham, Roudi Raheleh, Rakhshan Azadeh, Sabet Mehrdad Nasrollahzadeh, Shariftabrizi Ahmad, Madjd Zahra

机构信息

Oncopathology Research Center, Iran University of Medical Sciences, Tehran - Iran.

Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran - Iran.

出版信息

Int J Biol Markers. 2016 Feb 29;31(1):e53-61. doi: 10.5301/jbm.5000165.

DOI:10.5301/jbm.5000165

PMID:26391478

Abstract

INTRODUCTION

Skin cancers, particularly melanoma, are initiated and maintained by a subpopulation of tumor cells expressing stemness markers that are called cancer stem cells (CSCs). This study aimed to evaluate the expression levels and clinicopathological significance of the putative CSC markers CD44 and ALDH1A1 in patients with skin cancer.

METHODS

The expression levels of CD44 and ALDH1A1 were investigated in 107 skin cancer specimens including 58 (54%) basal cell carcinomas (BCC), 37 (35%) squamous cell carcinomas (SCC), and 12 (11%) melanomas using the tissue microarray (TMA) technique. The correlation of the expression levels of these markers and clinicopathological parameters was then analyzed.

RESULTS

The expression levels of CD44 and ALDH1A1 were significantly higher in melanoma patients than patients with SCC or BCC (p<0.001 and p = 0.002, respectively). A higher level of CD44 expression was more often found in melanoma tumor cells with a higher rate of recurrence (p = 0.029) and in SCC cases with ulceration (p = 0.01), while there was no significant correlation between ALDH1A1 expression and other clinicopathological parameters. Similarly, coexpression of CD44 and ALDH1A1 (CD44high/ALDH1A1high) was significantly observed in melanoma samples (p<0.001).

CONCLUSIONS

These findings suggest that a CD44high/ALDH1A1high phenotype in melanoma and a CD44high phenotype in SCC can be considered candidates for targeted therapy of skin cancers aiming at CSCs.

摘要

引言

皮肤癌，尤其是黑色素瘤，由表达干性标志物的肿瘤细胞亚群启动并维持，这些细胞被称为癌症干细胞（CSCs）。本研究旨在评估假定的CSC标志物CD44和ALDH1A1在皮肤癌患者中的表达水平及其临床病理意义。

方法

采用组织微阵列（TMA）技术，对107例皮肤癌标本进行研究，其中包括58例（54%）基底细胞癌（BCC）、37例（35%）鳞状细胞癌（SCC）和12例（11%）黑色素瘤。然后分析这些标志物的表达水平与临床病理参数之间的相关性。

结果

黑色素瘤患者中CD44和ALDH1A1的表达水平显著高于SCC或BCC患者（分别为p<0.001和p = 0.002）。在复发率较高的黑色素瘤肿瘤细胞（p = 0.029）和伴有溃疡的SCC病例（p = 0.01）中，更常发现较高水平的CD44表达，而ALDH1A1表达与其他临床病理参数之间无显著相关性。同样，在黑色素瘤样本中显著观察到CD44和ALDH1A1的共表达（CD44高/ALDH1A1高）（p<0.001）。