Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran.
BMC Cancer. 2020 Jun 17;20(1):567. doi: 10.1186/s12885-020-07050-6.
Skin cancer is the most common cancer worldwide and commonly classified into malignant melanoma (MM) and Nonmelanoma skin cancers (NMSCs), which mainly include basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). The extent to which Long Interspersed Element-1 (LINE-1, L1) ORF1p is expressed in cutaneous malignancies remains to be evaluated. This study aimed to assess LINE-1 ORF1p immunoreactivity in various skin cancer subtypes.
The expression level of LINE-1 ORF1p was evaluated in 95 skin cancer specimens comprising 36 (37.9%) BCC, 28 (29.5%) SCC, and 31 (32.6%) melanoma using the tissue microarray (TMA) technique. Then the association between expression of LINE-1 encoded protein and clinicopathological parameters was analyzed.
We showed that LINE-1 ORF1p expression level was substantially higher in BCC and SCC patients compared with melanoma samples (p < 0.001). BCC cases had a higher LINE-1 histochemical score (H-score) compared with SCC cases (p = 0.004). In SCC samples, a lower level of LINE-1 ORF1p expression was associated with age younger than the mean (p = 0.041). At the same time, no significant correlation was found between LINE-1 ORF1p expression and other clinicopathological parameters (all p > 0.05).
According to our observation, LINE-1 ORF1p immunoreactivity in various skin tumor subtypes extends previous studies of LINE-1 expression in different cancers. LINE-1ORF1p overexpression in NMSCs compared with MM can be considered with caution as a tumor-specific antigen for NMSCs.
皮肤癌是全球最常见的癌症，通常分为恶性黑色素瘤（MM）和非黑色素瘤皮肤癌（NMSC），主要包括基底细胞癌（BCC）和鳞状细胞癌（SCC）。长散布元件-1（LINE-1，L1）ORF1p 在皮肤恶性肿瘤中的表达程度仍有待评估。本研究旨在评估各种皮肤癌亚型中 LINE-1 ORF1p 的免疫反应性。
使用组织微阵列（TMA）技术评估 95 例皮肤癌标本中 LINE-1 ORF1p 的表达水平，包括 36 例（37.9%）BCC、28 例（29.5%）SCC 和 31 例（32.6%）黑色素瘤。然后分析 LINE-1 编码蛋白的表达与临床病理参数之间的关系。
我们表明，与黑色素瘤样本相比，BCC 和 SCC 患者的 LINE-1 ORF1p 表达水平明显更高（p<0.001）。BCC 病例的 LINE-1 组织化学评分（H 评分）高于 SCC 病例（p=0.004）。在 SCC 样本中，LINE-1 ORF1p 表达水平较低与年龄小于平均值有关（p=0.041）。同时，LINE-1 ORF1p 表达与其他临床病理参数之间未发现显著相关性（均 p>0.05）。
根据我们的观察，各种皮肤肿瘤亚型中 LINE-1 ORF1p 的免疫反应性扩展了 LINE-1 在不同癌症中表达的先前研究。与 MM 相比，NMSCs 中 LINE-1ORF1p 的过度表达可以谨慎地被认为是 NMSCs 的肿瘤特异性抗原。
