Kalantari Elham, Asgari Mojgan, Nikpanah Seyedehmoozhan, Salarieh Naghme, Asadi Lari Mohammad Hossein, Madjd Zahra
Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Hemmat Street (Highway), Next to Milad Tower, Tehran, 14496-14530, Iran.
Department of Pathology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Pathol Oncol Res. 2017 Oct;23(4):793-802. doi: 10.1007/s12253-016-0169-z. Epub 2017 Jan 12.
Cancer stem cells (CSCs) are the main players of prostate tumorigenesis thus; characterization of CSCs can pave the way for understanding the early detection, drug resistance, metastasis and relapse. The current study was conducted to evaluate the expression level and clinical significance of the potential CSC markers CD44 and CD133 in a series of prostate tissues. One hundred and forty eight prostate tissues composed of prostate cancer (PCa), high-grade prostatic intraepithelial neoplasia (HGPIN), and benign prostate hyperplasia (BPH) were immunostained for the putative CSC markers CD44 and CD133. Subsequently, the correlation between the expression of these markers and the clinicopathological variables was examined. A higher level of CD44 expression was observed in 42% of PCa, 57% of HGPIN, and 42% BPH tissues. In the case of CD133 expression PCa, HGPIN, and BPH samples demonstrated high immunoreactivity in 46%, 43%, and 42% of cells, respectively. Statistical analysis showed an inverse significant correlation between CD44 expression with Gleason score of PCa (P = 0.02), while no significant correlation was observed between CD133 expression and clinicopathological parameters. A significant reciprocal correlation was observed between the expression of two putative CSC markers CD44 and CD133 in PCa specimens while not indicating clinical significance. Further clinical investigation is required to consider these markers as targets of new therapeutic strategies for PCa.
癌症干细胞（CSCs）是前列腺肿瘤发生的主要参与者，因此，对癌症干细胞的特征描述可为理解早期检测、耐药性、转移和复发铺平道路。本研究旨在评估一系列前列腺组织中潜在癌症干细胞标志物CD44和CD133的表达水平及临床意义。对148份由前列腺癌（PCa）、高级别前列腺上皮内瘤变（HGPIN）和良性前列腺增生（BPH）组成的前列腺组织进行免疫染色，检测假定的癌症干细胞标志物CD44和CD133。随后，检查这些标志物的表达与临床病理变量之间的相关性。在42%的PCa、57%的HGPIN和42%的BPH组织中观察到较高水平的CD44表达。就CD133表达而言，PCa、HGPIN和BPH样本分别在46%、43%和42%的细胞中表现出高免疫反应性。统计分析显示，CD44表达与PCa的Gleason评分呈显著负相关（P = 0.02），而CD133表达与临床病理参数之间未观察到显著相关性。在PCa标本中，两个假定的癌症干细胞标志物CD44和CD133的表达之间观察到显著的相互相关性，但未显示出临床意义。需要进一步的临床研究，以将这些标志物视为PCa新治疗策略的靶点。
