Gandhi Jarel K, Zivkovic Lada, Fisher John P, Yoder Mervin C, Brey Eric M
Department of Biomedical Engineering, Wishnick Hall 223, 3255 South Dearborn Street, Chicago, IL 60616, USA.
Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade 11000, Serbia.
Sensors (Basel). 2015 Sep 18;15(9):23886-902. doi: 10.3390/s150923886.
Enhanced vascularization at sensor interfaces can improve long-term function. Fibrin, a natural polymer, has shown promise as a biomaterial for sensor coating due to its ability to sustain endothelial cell growth and promote local vascularization. However, the culture of cells, particularly endothelial cells (EC), within 3D scaffolds for more than a few days is challenging due to rapid loss of EC viability. In this manuscript, a robust method for developing fibrin microbead scaffolds for long-term culture of encapsulated ECs is described. Fibrin microbeads are formed using sodium alginate as a structural template. The size, swelling and structural properties of the microbeads were varied with needle gauge and composition and concentration of the pre-gel solution. Endothelial colony-forming cells (ECFCs) were suspended in the fibrin beads and cultured within a perfusion bioreactor system. The perfusion bioreactor enhanced ECFCs viability and genome stability in fibrin beads relative to static culture. Perfusion bioreactors enable 3D culture of ECs within fibrin beads for potential application as a sensor coating.
传感器界面处血管生成的增强可改善长期功能。纤维蛋白是一种天然聚合物，因其能够维持内皮细胞生长并促进局部血管生成，已显示出作为传感器涂层生物材料的潜力。然而，由于内皮细胞（EC）活力迅速丧失，在三维支架内培养细胞（尤其是内皮细胞）超过几天具有挑战性。在本论文中，描述了一种用于开发纤维蛋白微珠支架以长期培养封装内皮细胞的稳健方法。使用海藻酸钠作为结构模板形成纤维蛋白微珠。微珠的尺寸、溶胀和结构特性随针规以及预凝胶溶液的组成和浓度而变化。内皮集落形成细胞（ECFC）悬浮于纤维蛋白珠中，并在灌注生物反应器系统内培养。相对于静态培养，灌注生物反应器提高了纤维蛋白珠内ECFC的活力和基因组稳定性。灌注生物反应器能够在纤维蛋白珠内对内皮细胞进行三维培养，以作为传感器涂层进行潜在应用。
