Chen B, Bo C-J, Jia R-P, Liu H, Wu R, Wu J, Ge Y-Z, Teng G-J
Department of Urology, Southeast University School of Medicine, Nanjing, China.
Transplant Proc. 2013 Jun;45(5):2034-9. doi: 10.1016/j.transproceed.2013.01.096.
To investigate the renoprotective effects of exogenous endothelial progenitor cells (EPCs) on acute renal ischemia-reperfusion (I/R) injury in rats.
EPCs were collected by in vitro culture of mononuclear cells derived from rat bone marrow. The EPC labeling was performed using chloromethyl-benzamidodialkylcarbocyanine (CM-Dil). Sprague-Dawley rats were equally randomized into an I/R, an EPC, and a control group. We evaluated blood urea nitrogen (BUN) and serum creatinine (Scr), kidney morphology, apoptosis and microvessel density. EPC homing into I/R injured kidneys was observed using a fluorescence microscope.
After EPC transplantation, CM-Dil-labeled EPCs were noted at the corticomedullary junction of injured kidneys. The levels of BUN and Scr were markedly lower among the EPC than the I/R group (P < .05). The histopathologic score was higher in the I/R than the EPC group (P < .05). Apoptosis of tubular epithelial cells was substantially reduced among EPC-treated rats (P < .01). In addition, more CD34(+) microvessels were documented among the EPC than the other two groups (P < .01). The expression levels of vascular endothelial growth factor (VEGF) were also increased greatly in the EPC group (P < .05).
Transplanted exogenous EPCs exert protective effects on renal function by maintaining the integrity of peritubular capillaries after I/R injury.
探讨外源性内皮祖细胞（EPCs）对大鼠急性肾缺血再灌注（I/R）损伤的肾脏保护作用。
通过体外培养大鼠骨髓来源的单核细胞收集EPCs。使用氯甲基苯甲酰二烷基碳菁（CM-Dil）对EPCs进行标记。将Sprague-Dawley大鼠随机分为I/R组、EPC组和对照组。我们评估了血尿素氮（BUN）、血清肌酐（Scr）、肾脏形态、细胞凋亡和微血管密度。使用荧光显微镜观察EPCs归巢至I/R损伤的肾脏。
EPC移植后，在损伤肾脏的皮质髓质交界处观察到CM-Dil标记的EPCs。EPC组的BUN和Scr水平明显低于I/R组（P <.05）。I/R组的组织病理学评分高于EPC组（P <.05）。EPC处理的大鼠肾小管上皮细胞凋亡明显减少（P <.01）。此外，EPC组的CD34(+)微血管数量多于其他两组（P <.01）。EPC组血管内皮生长因子（VEGF）的表达水平也显著升高（P <.05）。
移植的外源性EPCs通过在I/R损伤后维持肾小管周围毛细血管的完整性对肾功能发挥保护作用。
