Liu Yu, Gong Ai-Jun, Qiu Li-Na, Li Jing-Rui, Li Fu-Kai
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Dapartment of Biotechnology, Daqing Branch of Heilongjiang Academy of Science, Daqing 163319, China.
Int J Environ Res Public Health. 2015 Sep 18;12(9):11829-47. doi: 10.3390/ijerph120911829.
The biodegradation effect and mechanism of decabromodiphenyl ether (BDE-209) by crude enzyme extract from Pseudomonas aeruginosa were investigated. The results demonstrated that crude enzyme extract exhibited obviously higher degradation efficiency and shorter biodegradation time than Pseudomonas aeruginosa itself. Under the optimum conditions of pH 9.0, 35 °C and protein content of 2000 mg/L, 92.77% of the initial BDE-209 (20 mg/L) was degraded after 5 h. A BDE-209 biodegradation pathway was proposed on the basis of the biodegradation products identified by GC-MS analysis. The biodegradation mechanism showed that crude enzyme extract degraded BDE-209 into lower brominated PBDEs and OH-PBDEs through debromination and hydroxylation of the aromatic rings.
研究了铜绿假单胞菌粗酶提取物对十溴二苯醚（BDE-209）的生物降解效果及机制。结果表明，粗酶提取物比铜绿假单胞菌本身表现出明显更高的降解效率和更短的生物降解时间。在pH 9.0、35℃和蛋白质含量为2000 mg/L的最佳条件下，5小时后初始BDE-209（20 mg/L）的92.77%被降解。基于气相色谱-质谱分析鉴定出的生物降解产物，提出了BDE-209的生物降解途径。生物降解机制表明，粗酶提取物通过芳香环的脱溴和羟基化将BDE-209降解为低溴代多溴二苯醚和羟基多溴二苯醚。
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